LC-MS/MS法测定氢氯噻嗪血药浓度的不确定度评价  

Evaluation of measurement uncertainty for the determination concentration of hydrochlorothiazide in human plasma by LC-MS/MS

作　　者：周洁[1] 刘江慧[1] 胡云芳[1] 程俊霖[1] 于翠霞[1] 余潜[1] 邹建军[1] 朱余兵[1] 樊宏伟[1] 

机构地区：[1]南京医科大学附属南京医院南京市第一医院临床药理实验室,南京210006

出　　处：《南通大学学报（医学版）》2013年第6期525-528,共4页Journal of Nantong University(Medical sciences)

摘　　要：目的:评价液相色谱-串联质谱联用法测定氢氯噻嗪血药浓度的不确定度,以便找到影响氢氯噻嗪浓度测定的不确定度的因素,为评价氢氯噻嗪浓度分析方法提供科学依据。方法:根据《测量不确定度评定与表示》和《化学分析中不确定度的评估指南》,评价液相色谱-串联质谱测定氢氯噻嗪血药浓度的不确定度。结果:30μg/L氢氯噻嗪的标准不确定度为0.99μg/L,扩展不确定度为1.98μg/L,质量浓度测定结果可表示为(31.32±1.98)μg/L。结论:建立的不确定评估方法可用于液相色谱-串联质谱联用法测定血浆中氢氯噻嗪浓度的不确定度评估。样品前处理和校正曲线拟合是主要的不确定度来源。测量不确定度的评定方法的确立对于血浆中氢氯噻嗪浓度测定方法标准的研究具有重要意义。Objective：To evaluate the uncertainty in the determination of hydrochlorothiazide in human plasma by LC- MS/MS and find out the influencing factors to provide scientific basis for the analytical method. Methods： The uncertainty in the determination of hydrochlorothiazide in human plasma by LC-MS/MS was analyzed and evaluated according to the“Evaluation and Expression of Uncertainty Measurement”and the “Manual of Uncertainty Evaluation in Chemical Analysis”.Results：The standard uncertainty of hydrochlorothiazide in human plasma with the dosage of 30μg/L was 0.99μg/L, and the expand uncertainty was 1.98 μg/L, the result of the determination could be expressed as（31.32＆#177;1.98） μg/L. Conclusion：The uncertainty of this experiment was mainly caused by the sample pretreatment and the calibration curve. The established uncertainty assessment method can be used in the uncertainty evaluation in the determination of hydrochlorothiazide in human plasma by LC-MS/MS and is valuable in the determination standard study of hydrochlorothiazide in plasma.

关 键 词：氢氯噻嗪 血药浓度 不确定度评价 液相色谱-串联质谱 

分 类 号：R965[医药卫生—药理学]