216例金黄色葡萄球菌中红霉素对克林霉素诱导耐药检测  

Detection of erythromycin-induced resistance to clindamycin among 216 strains of staphylococcus aureus

作　　者：胡丽娟[1] 刘华山 陈淑娟[1] 

机构地区：[1]福建省莆田学院附属医院检验科,351100 [2]福建省莆田市城厢区医院

出　　处：《中国实用医药》2013年第36期36-37,共2页China Practical Medicine

摘　　要：目的了解金黄色葡萄球菌(SA)对红霉素和克林霉素的耐药性,检测红霉素对克林霉素诱导耐药的发生率。方法采用美国临床实验室标准化协会(CLSI)推荐的纸片扩散法,用红霉素和克林霉素双纸片法(D-试验)和VITEK 2 Compact AES分析红霉素对克林霉素诱导耐药表型。结果 216株SA中有76株(MSSA22株,MRSA54株)呈红霉素耐药、克林霉素敏感,其中29株D-试验阳性(MSSA8株,MRSA 21株),检出率分别为36.4%、38.9%。结论临床微生物实验室应加强对SA中克林霉素诱导耐药的检测,以指导临床医生合理选用大环内酯类、林可酰胺类抗菌药物。Objective To investigate the resistance of staphylococcus aureus （SA） to erythromycin and clindamycin, and detect the incidence of erythromycin-induced resistance to clindamycin. Methods Disk diffusion susceptibility method was performed for erythromycin and clindamycin according to the Clinical and Laboratory Standards Institute （CLSI）. The inducible resistance phenotype was analyzed by clindamycin and erythyomycin double-disk diffusion method （D test） and VITEK 2 Compact AES. Results Analyzing the susceptibility of 216 SA strains, 76 strains （22 strains of MSSA, 54 strains of MRSA） were resistant to erythromycin but sensitive to clindamycin. 29 of the 76 strains were positive in D-test （8 strains of MSSA, 21 strains of MRSA）;the positive rates were 36.4%and 38.9%respectively. Conclusion The occurrence of induced resistance to clindamycin is considerably high. It suggested that D-test should be routinely performed in erythromycin-resistant but clindamycin-sensitive staphylococcus in microbiological laboratories. The report of inducible clindamycin resistance is significant evidence to guide clinical physicians to use antibiotic correctly.

关 键 词：金黄色葡萄球菌 红霉素 克林霉素 诱导耐药 D-试验 

分 类 号：R446.5[医药卫生—诊断学]