淫羊藿女贞子配伍对GIOP大鼠骨代谢生化指标的影响研究  

Study of Effects of Combination of Herba Epimedii and Fructus Ligustri Lucidi on Biochemical Indicators in Glucocorticoid-induced Osteoporosis Rats

作　　者：刘仁慧[1] 康学[1] 张伟华[2] 周琦[3] 王秀娟[1] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]上海中医药大学附属岳阳中西医结合医院中医科,上海200437 [3]首都医科大学附属北京中医医院内科门诊,北京100010

出　　处：《世界中医药》2013年第12期1450-1453,共4页World Chinese Medicine

基　　金：北京中医药薪火传承"3+3"工程二室一站建设项目(编号:2012-SZ-C-42);首都中医药及护理学研究专项课题(编号:12ZYH07);北京市教育委员会科技计划面上项目(编号:KM201310025013)

摘　　要：目的:探讨淫羊藿、女贞子对糖皮质激素致骨质疏松(Glucocorticoid-induced Osteoporosis,GIOP)模型大鼠骨代谢生化指标的影响。方法:Sprague Dawley(SD)大鼠60只,雌雄各半,随机分为正常对照组、模型对照组、女贞子组、淫羊藿组、淫羊藿女贞子组、钙尔奇组。每周两次肌肉注射地塞米松(1 mg/kg)建立骨质疏松模型。造模同时各给药组灌服相应药物,8周后检测血清钙(Ca)、磷(P)、碱性磷酸酶(alkalineph-osphatase,AKP)、抗酒石酸酸性磷酸酶(tartrate-resistant acidphosphatase,StrACP)、骨钙素(Bone Gla-protein,BGP)及骨Ca、P含量。结果:模型对照组较正常对照组血清AKP、StrACP活性以及血P含量均显著升高,血清BGP、血及骨Ca含量均显著下降;与模型对照组比较,各给药组均可显著下调血清AKP、StrACP水平,提高血清BGP含量;且可不同程度地纠正血及骨Ca、P代谢紊乱。结论:淫羊藿、女贞子配伍可明显改善骨代谢生化指标,有效纠正激素诱导骨质疏松模型大鼠骨代谢异常,从而发挥抗骨质疏松作用。Objective： To explore the effects of combination of Herba Epimedii and Fructus Ligustri Lucidi on biochemical indicators in glucocorticoid-induced osteoporosis rats. Methods： A total of 60 SD rats were randomly divided into 6 groups： normal control group,model control group,Herba Epimedii group,Fructus Ligustri Lucidi group,Herba Epimedii and Fructus Ligustri Lucidi mixture group,and positive drug group. All rats received intramuscular injection of dexamethasone（ 1mg / kg,twice a week） for eight weeks to establish osteoporosis model（ except those in the control group）. Rats in treatment groups were treated with intragastric administration of traditional Chinese herbal decoction or positive drug respectively. Eight weeks later,serum biochemical indexes,including osteocalcin（ BGP）,calcium（ Ca）,phosphorus（ P）,alkaline phosphatase（ AKP）,tartrate-resistant acid phosphatase（ StrACP）,and the content of bone Ca and bone P were tested. Results： Serum AKP and StrACP activity and the concentration of serum P in model group were significantly higher than that of the normal control group,while the content of serum BGP,serum Ca and bone Ca were obviously lower. Compared with the model group,serum AKP and StrACP activity significantly decreased and serum BGP content increased significantly in each administration group,and serum and bone Ca and P metabolish disorders were corrected in varying degrees. Conclusion： Combination of Herba Epimedii and Fructus Ligustri Lucidi can significantly improve biochemical indicators and effectively correct abnormality of bone metabolism in glucocorticoid-induced osteoporosis rats,thus plays an important role in anti-osteoporosis.

关 键 词：骨质疏松 糖皮质激素 淫羊藿 女贞子 骨代谢 生化指标 

分 类 号：R681[医药卫生—骨科学]