中药提取物AFDR对IBRC材料复合ADMSCs应用于兔腰椎横突间融合的影响  

Research on the Impact of Herbal Extracts AFDR Combined with IBRC and ADMSCs used for Rabbit Lumbar Transverse Interbody Fusion

作　　者：李晋玉[1] 徐林[1] 金合 谭荣伟[3,4] 俞兴[1] 

机构地区：[1]北京中医药大学东直门医院骨科中心,北京100700 [2]北京市延庆中医院骨科,北京102100 [3]清华大学材料学院,北京100086 [4]深圳兰度生物材料有限公司,深圳518057

出　　处：《世界中医药》2013年第12期1454-1457,共4页World Chinese Medicine

基　　金：北京中医药大学青年教师资助计划;教育部新世纪优秀人才支持计划基金项目(编号:NCET-10-0249)

摘　　要：目的:本实验采用可注射骨修复材料(Injectable Bone Regeneration Composite,IBRC)复合脂肪间充质干细胞(Adipose Tissue-Derived Mesenchymal Stromal cells,ADMSCs)应用于兔腰椎横突间融合模型,骨碎补总黄酮(Assemble Flavone of Drynaria Rhizome,AFDR)灌胃,观察融合效果。方法:60只成年白兔,制成兔腰椎横突间融合模型,采用安全随机法随机分为5组:A组植入IBRC材料结合AFDR灌胃;B组植入IBRC材料复合自体髂骨(Autologous iliac Crest Bone,ACB),结合去离子水﹙Deionized Water,DW﹚灌胃;C组植入IBRC材料+ADMSCs,DW灌胃;D组,植入ACB,DW灌胃;E组植入ACB,AFDR灌胃。以术后第8周为观察时间点,进行大体观测,手触检测,X线片,组织学观察。结果:IRBC+ADMSCs,ACB+AFDR组获得较好的融合率,显著高于单纯IRBC,ACB组(P<0.05),与ACB+DW组相比差异无统计学意义(P>0.05)。结论:自体ADMSCs结合IRBC材料可实现与ACB组相似的融合率,结合中药AFDR灌胃,能够提高兔腰椎横突间的骨性融合率。Objective： To explore the effect of Adipose Tissue-Derived Mesenchymal Stromal cells（ ADMSCs） which is Injectable Bone Regeneration Composite（ IBRC） combined with intra-gastric administration of Assemble Flavone of Drynaria Rhizome（ AFDR） on rab- bits with lumbar transverse interbody fusion. Methods： Sixty adult rabbits were made into rabbit lumbar transverse interbody fusion mod- els,randomly divided into five groups including A group（ IBRC ＋ AFDR）,B group（ IBRC ＋ Autologous iliac Crest Bone（ ACB） ＋ Dei- onized Water（ DW））,C group（ IBRC ＋ ADMSCs ＋ DW）,D group（ ACB ＋ DW）,E group（ ACB ＋ AFDR）. After eight weeks of treat- ment,specimens were collected for gross observation,touch detection,X-ray,histological observation. Results： The fusion rates of group C and group E were significantly higher than that of group B（ P 0. 05）,and there was no significantly statistical difference（ P 0. 05） compared with the group D. Conclusion： IRBC composite ADMSCs can achieve a similar fusion rate as ACB group. Combing with tradi- tional Chinese AFDR can improve the rate of the intertransverse lumbar fusion in rabbits.

关 键 词：脂肪间充质干细胞 兔 腰椎横突间融合 可注射骨修复材料 骨碎补总黄酮 

分 类 号：R681.5[医药卫生—骨科学]