增液汤干预干燥综合征模型鼠AQP1/AQP8的表达规律研究  

Intervention of Zengye Decoction on Expression of Aquaporin-1,8 in the Mice with Sjogren's Syndrome

作　　者：吴晓丹[1] 任长虹[2] 张林[1] 周洪伟[3] 程涓[4] 杨勇[1] 

机构地区：[1]北京中医药大学,北京100029 [2]首都医科大学宣武医院,北京100053 [3]中国中医科学院,北京100700 [4]北京中医药大学东直门医院,北京101121

出　　处：《世界中医药》2013年第12期1461-1464,共4页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金资助项目(编号:81001495/H2705)

摘　　要：目的:观察增液汤干预下干燥综合征(Sjogren's Syndrome,SS)模型小鼠肺及结肠组织中水通道蛋白AQP1(aquaporin1)、AQP8(aquaporin8)的表达规律。方法:选用BALB/c小鼠,各组10只,免疫诱导法建立干燥综合征小鼠模型,产生SS特异性临床表现和病理改变,采用免疫组化和Western blot蛋白质印迹法观察增液汤对SS模型小鼠肺及结肠组织中AQP1/AQP8表达的影响。结果:增液汤可以增强肺及结肠组织中AQP1的蛋白表达(P<0.01),对AQP8无影响。结论:AQP1在干燥综合征模型鼠多组织中的表达下调,可能是干燥综合征水液代谢障碍,多脏器损伤的机制之一;增液汤恢复机体阴液,起到"增水"功效可能与上调AQP1蛋白表达有关。Objective： To observe the expressions of AQP1 and AQP8 in lung and colon tissue of mice with Sjogren＇s syndrome. Meth- ods： Fifty BALB /c mice were randomized into five groups,with10 mice in each group. The mice models with Sjogren＇s syndrome were es- tablished by immune inducing method. It was able to produce the specific clinical symptoms and pathological changes of Sjogren＇s syn- drome. The effect of Zengye decoction on the expression of AQP1 / AQP8 in lung and colon tissue was observed by immunohistochemistry and Western blot. Results： Zengye decoction can strengthen the AQP1 protein expression in lung and colon tissue（ P 0. 01）,however AQP8 was barely changed. Conclusion： It could be one of the causes of Sjogren＇s syndrome that AQP1 expression was reduced in multi- ple organ in the mice with Sjogren＇s syndrome. Zengye decoction can increase the body fluid,and the effect may be associated with the increased AQP1 expression.

关 键 词：AQP1 AQP8 干燥综合征 WESTERN BLOT 增液汤 

分 类 号：R442.8[医药卫生—诊断学]