孵育和激活剂作用时间对山羊核移植胚胎早期发育的影响  

Effects of incubation time and activation time of 6-DMAP on the early development of the goat nuclear transplanted embryos

作　　者：李斌[1,2] 曹陈冲[2] 陈立子[2] 汪存利[2] 赵焕[2] 李东伟[2] 陶勇[2] 章美林[2] 章孝荣[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院,上海200011 [2]安徽农业大学动物科技学院,合肥233036

出　　处：《安徽农业大学学报》2014年第1期10-13,共4页Journal of Anhui Agricultural University

基　　金：上海高校选拔培养优秀青年教师科研专项基金(zzjdyx12106);安徽省"十五"重大科技专项(04003005);"十一五"科技攻关项目(06013051A)共同资助

摘　　要：探讨了电融合前对重构卵继续孵育及激活剂6-DMAP不同作用时间对山羊核移植胚胎体外发育的影响。结果显示，重构卵在成熟液中继续培养后，9～10h组的融合后卵子死亡率（27．42％）显著低于30min和90min组（49．26％和56.3％）（P〈0．05），胚胎卵裂率（68．15％）则显著高于30min和90min组（55．6％和54．24％）（P〈0．05）；重构卵用离子霉素激活后，再用6-DMAP激活0h、2h、3h、4h、5h及8～10h，各组间的胚胎卵裂率均无显著差异（P〉0．05），但均显著高于未用6-DMAP组的卵裂率（氏0．05）。本试验结果表明，电融合前，继续成熟培养重构卵有利于降低电融合时的卵子死亡率，提高卵子利用率；联合使用离子霉素和6-DMAP有利于提高激活效果，而6一DMAP的作用时间（2～10h）不影响核移植胚胎的卵裂。The study was conducted to examine the effects of incubation time before the electrofusion and activation time of 6-DMAP on the development of the goat nuclear transplanted embryos in Vitro. The recon- structed oocytes were continually cultured in a maturation medium for 30 min, 90 rain and 9-10 h before the elec-trofusion, respectively. The results showed that the mortality of reconstructed oocytes of 9-10 h group after the electrofusion was significantly lower than those of 30 min and 90 min groups （27.42% vs. 49.26% and 56.3%, P〈0.05）, while the cleavage rate of 9-10 h group was significantly higher than those of 30 min and 90 min groups （68.15% vs. 55.6% and 54.24%, P〈0.05）. The nuclear transplanted embryos activated by ionomycin were activated with 6-DMAP for 0 h, 2 h, 3 h, 4 h, 5 h, and 8-10 h respectively. The results showed that the cleavage rate was not significantly different among groups （P〉0.05）, but it was significantly higher than that of the embryos without 6-DMAP （P〈0.05）. In conclusion, the continued incubation of reconstructed oocytes before electrofusion may be helpful for lowing the mortality of fusion and increasing the rate of available oocyte; the combination of ionomycin with 6-DMAP could improve the activation efficiency in goat nuclear transplanted embryos, and activation time of 6-DMAP has no significant effects on the cleavage of goat nuclear transplanted embryos.

关 键 词：山羊 重构卵 核移植胚胎 化学激活 6-DMAP 

分 类 号：S828.36[农业科学—畜牧学]