超低温冷冻对德国牧羊犬顶体酶类活性的影响  

The effect of cryopreservation on acrosomal enzyme activity in German shepherd dog

作　　者：沈留红[1] 宗小兰[2] 杨庆稳[3] 邓俊良[1] 王海光[4] 曹随忠[1] 余树民[1] 张建勇 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]四川农业大学教务处,四川雅安625014 [3]重庆三峡职业学院动物科技系,重庆404155 [4]中国兽医药品监察所,北京100081 [5]雅安雨城区农业局,四川雅安625014

出　　处：《黑龙江畜牧兽医》2014年第2期4-7,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31172379);四川省教育厅自然科学重点项目(2006A009);四川省科技厅应用基础研究项目(2008JY0098);四川农业大学优秀学士论文培育计划资助项目(00209050)

摘　　要：为了探讨超低温冷冻对德国牧羊犬透明质酸酶(HYD)、顶体酶(ACE)和酸性磷酸酶(ACP)活性的变化,试验分别对冷冻前后德国牧羊犬精子采用改良明胶底膜法、改良BNPNA法、磷酸苯二钠法对精子HYD、ACE和ACP活性进行研究。结果表明:超低温冷冻前后精子HYD、ACE和ACP活性极显著降低(P<0.01);解冻后精子3种顶体酶类活性分别与精子活率和顶体完整率呈正相关,其中ACE活性与顶体完整率呈极显著正相关,相关系数为0.487(P<0.01)。3种顶体酶类活性与畸形率均呈显著负相关(P<0.05),ACE活性与精子顶体完整率的相关系数为0.487,相关度大于HYD(0.439和0.459)和ACP(0.440);而HYD活性与精子活率的相关系数为(0.404和0.423),相关度大于ACE(0.377)和ACP(0.369)。说明超低温冷冻使德国牧羊犬精子3种顶体酶类活性均显著降低;3种顶体酶类活性分别与精子活率和顶体完整率呈正相关,与畸形率呈负相关。To investigate the effects of cryopreservation on the activities of hyaluronidase （HYD）, acrosome enzyme （ACE）, and acid phosphatase （ACP） in German shepherd dog, the modified gelatin - substrate film technique, the modified BAPNA method , and disodium phenyl phosphate method were used to determine the sperm HYD, ACE and ACP activities of German shepherd dog before and after freezing, respectively. The results showed the sperm HYD, ACE and ACP activities were significant reduced （P 〈 0.01 ） both before and after cryopreservation. The activities of the three kinds of acrosomal enzymes for the thawed sperm were positively correlated with sperm motility and acrosome integrity, in which the ACE activity had a highly significant positive correlation （ P 〈0.01 ） with the acrosome integrity and the correlation coefficient was r = 0. 487. The activities of the three kinds of acrosomal enzymes had highly significant negative correlations （ P 〈 0.05 ） with the aben＇ation rate. The correlation coefficient between the ACE activity and sperm acrosome integrity was 0. 487, which was greater than that of HYD （0. 439 and 0. 459 ） and ACP（0.440）, while the correlation coefficients between the HYD activity and the sperm motility rate were 0. 404 and 0. 423 respectively, which were greater than those of ACE （0. 377 ） and ACP （0. 369） respectively. The results indicate that the HYD, ACE and ACP activities are significantly reduced by cryopreservation. The activities of the three kinds of acrosomal enzymes have positive correlations with the sperm motility and acrosome integrity, showing negative correlations with the aberration rate.

关 键 词：超低温冷冻 德国牧羊犬 透明质酸酶 顶体酶 酸性磷酸酶 精子 

分 类 号：S829.2[农业科学—畜牧学]