胞质雄性不育新基因T1243在大白菜中的遗传转化  

A Novel Cytoplasmic male Sterility Gene T1243 Expressed in Transgenic Chinese Cabbage

作　　者：吴玲玲[1] 刘志强[1] 张丽萍[1] 乔增杰[1] 陈竹君[2] 裴雁曦[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]浙江大学农业与生物技术学院园艺系,浙江杭州317400

出　　处：《山西大学学报（自然科学版）》2014年第1期121-126,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(31071809)

摘　　要：以大白菜带柄子叶为外植体,通过农杆菌介导法,优化大白菜的遗传转化体系,得出结论:津育75的最佳筛选培养基为MS+2.0mg/L 6-BA+0.3mg/L NAA+7.0mg/L AgNO3+7.5mg/L Kan+200mg/L Amp,外植体在黑暗条件下预培养2d,然后用OD600值为0.1菌液侵染2min,共培养2d时转化率最高,为35.48%.对抗性植株进行分子检测,结果表明T1243基因已整合到大白菜中,并可以正常表达。Cotyledons with petiole were used as explants, and the culture conditions of transformed plants mediated by Agrobacterium turnefaciens were optimized. The results showed that optimum organization culture medium for Jinyu 75 was MS＋2.0 mg/L 6-BA ＋ 0.3 mg/L NAA＋7.0 mg/L AgNO3 ＋7.5 mg/L Kan@200 mg/L Amp. The optimum time of pre-culturing is for 2 d in darkness,and the highest transform frequency is 35.48% when explants are immersed in bacterium concentration of OD-600=0. 1 for 2 min. Re- suits from PCR and RT-PCR showed that T1243 was integrated into Chinese cabbage successfully and it was expressed normally.

关 键 词：大白菜 雄性不育基因 遗传转化 转基因植株 

分 类 号：Q785[生物学—分子生物学]