替米沙坦对血管紧张素Ⅱ诱导的血管内皮细胞氧化/还原失衡的保护作用  

Protective Effect of Telmisartan Against Oxidation-reduction Disequilibrium of Vascular Endothelial Cells Induced by Angiotensin Ⅱ

作　　者：郑师明[1] 黄汉辉[1] 刘少志[1] 梁颖[1] 张欣[1] 严鹏科[1] 

机构地区：[1]广州医科大学附属第三医院药学部,广州510150

出　　处：《中国现代应用药学》2014年第1期22-26,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的探讨血管紧张素Ⅱ(AngⅡ)对血管内皮细胞氧化/还原平衡的影响及替米沙坦的保护作用。方法人大动脉血管内皮细胞株接种于35 mm玻璃底细胞培养皿中,随机分成空白对照组、AngⅡ模型组及3个浓度替米沙坦试验组(60,300,1 000μg·L-1),每组42例样本。测定不同取样时间点血管内皮细胞SOD活性和MDA含量。结果血管内皮细胞受AngⅡ刺激后,细胞MDA含量瞬时激增,SOD活性受到抑制,替米沙坦预处理过的血管内皮细胞能有效抵御AngⅡ诱发的氧化应激反应,提高SOD活性和抑制MDA含量的升高,并呈现替米沙坦剂量依赖性关系。结论替米沙坦能够抑制AngⅡ诱导的细胞氧化应激反应,从多方面保护动脉血管。OBJECTIVE To explore the effect of angiotensin Ⅱ on oxidation-reduction equilibrium system of vascular endothelial cell and antagonism of Telmisartan. METHODS After primary cultured on 35mm glass bottom cell culture dishes, the Human vascular endothelial cell randomly divided into 5 experimental groups which were blank control group, angiotensin II model group and three different concentration of telmisartan in treatment groups（60μg/L、300μg/L、1000μg/L）.Each group contents 42 samples. The concentration of MDA and the activity of SOD in vascular endothelial cell were detected in different sampling time. RESULT The concentration of MDA was proliferate instantaneous and the activity of SOD was restrained after Ang II stimulation.The Vascular endothelial cells after Telmisartan pretreatment can significantly resist the oxidative stress induced by Ang II. Furthermore, the time-dosage dependent relationship existed in the protection of Telmisartan. CONCLUSION Telmisartan can resist the oxidative stress induced by Ang II.

关 键 词：替米沙坦 血管紧张素Ⅱ 氧化应激反应 

分 类 号：R965.2[医药卫生—药理学]