中国7个地区诺如病毒GⅡ．4／Sydney2012变异株分析  

Study on norovirus G Ⅱ .4/Sydney 2012 variant in China

作　　者：买欢 高燕[1] 潘孝本[2] 韩进超[2] 丛旭[2] 魏来[1] 

机构地区：[1]北京大学人民医院感染科,100044 [2]北京大学人民医院肝病研究所,100044

出　　处：《中华流行病学杂志》2014年第2期157-162,共6页Chinese Journal of Epidemiology

摘　　要：目的分析2012--2013年冬季中国地区诺如病毒新流行株GⅡ．4／Sydney2012的衣壳蛋白区核苷酸与氨基酸变异特点。方法对已获得的22份2012--2013年冬季北京地区GⅡ．4／Sydney2012株进行全衣壳蛋白区基因扩增。从GenBank检索中国其他地区的GⅡ．4／Sydney2012株衣壳蛋白区核苷酸序列。对获得的GⅡ．4／Sydney2012株的衣壳蛋白区核苷酸及氨基酸序列与往年GⅡ．4流行株进行系谱分析。结果获得中国7个地区（北京、上海、江苏、湖州、荆州、香港和台湾）的GⅡ．4／Sydney2012株资料共38份（至少包含完整VPl核苷酸序列）。GⅡ．4／Sydney2012株之间VPI核苷酸差异为0．1％～3．3％，氨基酸差异为0—3．1％。系谱分析发现GⅡ．4／Sydney2012株与Apeldoom2008和NewOrleans2009起自共同的分支。中国和悉尼GⅡ．4／Sydney2012代表株的A、D、E抗原表位氨基酸序列组合有两种：TSRN—GTT-SNT和TSRN．STT-SNT。结论GⅡ．4／Sydney2012株已在中国多个地区出现，各地区病毒株同源性较高。GⅡ．4／Sydney2012株的抗原表位氨基酸组合发生明显改变。Objective To study the nucleotide and amino acid sequences of norovirus G Ⅱ.4/ Sydney 2012 variants, in China. Methods Twenty-two stool specimens, confirmed as G Ⅱ .4/Sydney 2012- positive were collected from Beijing in the winter of 2012-2013. RT-PCR was performed to target the complete capsid gene. G Ⅱ.4/Sydney 2012 strains from other regions in China were searched and obtained from the GenBank. Nucleotide and amino acid sequences of G Ⅱ A/Sydney 2012 strains were analyzed, using the CLUSTAL X （Version 1.83）and followed by phylogenetic analysis using Mega version 5.1. Results The complete major capsid nucleotide sequences of thirty-eight G Ⅱ .4/ Sydney 2012 strains from seven regions in China were obtained. The VP1 nucleotide and amino acid sequences diversity were 0.1%-3.3% and 0-3.1%, respectively. Result from phylogenetic analysis demonstrated that the G Ⅱ A/Sydney 2012 variant shared a common ancestor with both the dominant norovirus GⅡ .4 variants Apeldoom 2008 and the New Orleans 2009. G ⅡA/Sydney 2012 variants appeared to have had two A/D/E site combinations at the amino acid level, TSRN-GTT-SNT and TSRN-STT-SNT. Conclusion G lI A/Sydney 2012 variant had been circulating in many regions in China. There seemed a high nucleotide and amino acid identity among G Ⅱ A/Sydney 2012 strains collected from China. G Ⅱ A/Sydney 2012 variants showed different A/D/E site combination from other pandemic G Ⅱ .4 variants.

关 键 词：诺如病毒 GⅡ 4基因型 变异株 衣壳蛋白基因 

分 类 号：R373[医药卫生—病原生物学]