云南省鼠疫菌FseI酶切分型及其流行病学意义  

Genotyping and its epidemiological significance on Yunnan Yersinia pestis under Fse I enzyme digestion method

作　　者：石丽媛[1] 叶蕊[2] 董珊珊[1] 郭英[1] 杨光璨[1] 张蓉[2] 崔志刚[3] 李伟[3] 王鹏[1,2] 

机构地区：[1]云南省地方病防治所云南省鼠疫防控技术重点实验室,大理671000 [2]大理学院公共卫生学院 [3]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室

出　　处：《中华流行病学杂志》2014年第2期182-185,共4页Chinese Journal of Epidemiology

基　　金：国家自然科学基金（81160354）;卫生部行业基金（201202021）;云南省应用基础研究（2011FB134）

摘　　要：目的构建云南省鼠疫菌FseI酶切的脉冲场凝胶电泳（PFGE）图谱库并探讨其流行病学意义。方法采用限制性内切酶FseI对云南家、野鼠型及丽江玉龙鼠疫菌株进行酶切分析，并聚类分析电泳图谱。结果30株被试菌株分为16种PFGE型别，其相似性系数为79．8％～100．O％；16种PFGE基因型可以分为4个簇，除EV76自成一簇，其余3个簇分别为家鼠型基因簇、野鼠型基因簇及玉龙基因簇。结论云南省鼠疫菌PFGE基因型与生态型、生物型相吻合，具有一定的区域聚集性，而丽江玉龙鼠疫菌为一个独立基因簇。Objective To create the fingerprint library ofYunnan Yersinia pestis by pulse field gel electrophoresis （PFGE） with Fse ｜ enzyme digestion method and to study its epidemiological significance. Methods We used rare cutting restriction enzyme Fse I to digest Yunnan Yersinia pestis strains that were isolated from foci including Rattus flavipectus Plague Focus, Apodemus chevrieri- Eothenomys miletus Plague Focus and Yulong Plague Focus. Fingerprints to Bionumerics software were used for cluster analysis. Results 30 tested strains were divided into 16 genotypes with the similarity value as 79.8%-100.0%. 16 genotypes wee formed into 4 clusters, with one consisted of only EV76 while the other three belonged to Rattusflavipectus ,Apodemus chevrieri- Eothenomys miletus and Yulong clusters, respectively. Conclusions PFGE genotypes of Yurman Yersinia pestis accorded with its ecotypes and biovars, with clustered regional features. The strains isolated from Yulong showed an unique PFGE type, indicating a new clone group was identified.

关 键 词：鼠疫耶尔森菌 脉冲场凝胶电泳 FseI酶切方法 

分 类 号：R378[医药卫生—病原生物学]