光质对离体诱导棕色棉纤维基因表达影响的cDNA-SRAP分析  

Differential Expression Analysis of the Effect of Light Quality on Tissue Culture of Brown Cotton Fiber by cDNA-SRAP

作　　者：陈沙沙[1] 樊洪泓[1] 关蕾[1] 林毅[1] 蔡永萍[1] 钱森和[1,2] 孙旭[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]安徽工程大学,安徽芜湖241000

出　　处：《棉花学报》2014年第1期34-40,共7页Cotton Science

基　　金：安徽省教育厅重点项目(KJ2011A103);安徽省棉花产业创新体系

摘　　要：以离体培养棕色棉纤维(暗培养10 d)为材料,分别用红、黄、蓝、白光进行处理,在24 h光照条件下培养10d后,利用cDNA-SRAP技术对其基因表达进行分析。对其中16个差异片段测序后进行同源性比对和功能分析表明:2个序列功能与植物抗逆性有关(CNGC5类似蛋白基因、(+)-δ-杜松烯合成酶);3个序列与生物代谢相关(β-1,3-葡聚糖酶13基因、质膜胆碱转运蛋白基因、tau类谷胱甘肽转移酶基因)。其中,谷胱甘肽转移酶基因与原花青素合成前体物质的转运有关,光质可能通过影响谷胱甘肽转移酶基因进而对原花青素的合成产生影响。Fibers of brown cotton were treated with different light qualities （red, yellow, blue, and white） for 10 days in 24-h light conditions, after which there gene expressions were analyzed using the cDNA-SRAP technique. The biological functions of 16 differentially expressed genes were predicted and characterized.The results showed that two fragments had high sequence similarity with resistance genes （CNGC5-like protein gene, （＋）-delta-cadinene synthase （cdn1-C6） pseudogene）, 3 sequence related to biological metabolism （sugar transporter/spinster transmembrane protein （PtrOATP2））, beta-1,3-glucanase 13（B13G_13） mRNA, and tau class glutathione transferase GSTU43 ）. Among them, the glutathione transferase was associated with the synthesis of proanthocyanidins. Light quality may affect glutathione transferase, thereby affecting the synthesis of proanthocyanidins.

关 键 词：离体培养 cDNA—SRAP 谷胱甘肽转移酶 植物抗逆性 

分 类 号：S562[农业科学—作物学]