陆地棉种子发育过程中microRNA的挖掘与功能研究  

Identification of microRNAs in Upland Cotton

作　　者：Wang Gaskin 余道乾[1] 杜雄明[1] 

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳45500

出　　处：《棉花学报》2014年第1期81-86,共6页Cotton Science

基　　金：国家"十一五"科技支撑计划项目(2006BAD13B04);国家"973"计划项目(2004CB117301)

摘　　要：microRNAs(miRNAs)广泛参与调控植物的生长和发育,但这类分子是否对棉花胚珠和纤维的生长发育起到重要的作用,还有待验证。对这些小分子的认识,一般先要从序列信息的获得上开始。应用基因芯片对TM-1+5 DPA(开花后5 d)胚珠总RNA进行了miRNA的筛选研究。结果显示,含有352个探针的芯片成功钓取到199个陆地棉miRNA,其中绝大多数为首次报道。此外,对miR399研究发现,它与纤维中磷的含量变化相关;miR169可能与干旱应激反应有关。MicroRNAs（miRNAs） are involved in the regulation of plant growth and development, but whether such molecules participate in cotton ovule or fiber growth or development is yet to be verified. Generally, understanding of these small molecules begins by obtaining sequence information. To clone miRNAs in cotton, an oligonucleotide microarray was used to screen total RNA of ＋5 days post anthesis TM-1 ovules. The microarray bad 352 known plant miRNA probes. Ultimately, 199 miRNAs were probed. In addition, we found that miR399s is related to the phosphorus content of the cotton fiber, and miR169s might play a role under drought stress in cotton.

关 键 词：陆地棉 基因芯片 MIRNA 茎环RT-PCR 

分 类 号：S562[农业科学—作物学]