生物滴滤塔处理苯酚气体研究  

Gaseous Phenol Removal in a Bio-Trickling Filter

作　　者：何觉聪[1] 黄倩茹[1] 陈洲洋 叶杞宏[1] 罗雨薇[1] 魏在山[1] 

机构地区：[1]中山大学环境科学与工程学院、广东省环境污染控制与修复技术重点实验室,广州510275

出　　处：《环境科学》2014年第2期520-525,共6页Environmental Science

基　　金：广东省科技计划项目(2011A030700010);广州开发区科技计划重点科技攻关计划项目(2010Q-P161);企业委托项目(7102651,7101614)

摘　　要：采用生物滴滤塔处理苯酚气体,考察了苯酚去除性能的影响因素.结果表明,生物滴滤塔能高效处理苯酚气体,苯酚去除效率可达99.5%,长期运行平均去除效率在98%左右.适宜的运行条件为:停留时间20.6 s,循环液pH值7.0,喷淋密度1.67 m3·(m2·h)-1.采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术研究处理苯酚气体的生物滴滤塔填料表面的微生物,结果表明,生物滴滤塔内有5种降解苯酚的优势菌种:Polaromonas sp.、Acinetobacter sp.、Acidovorax sp.、Veillonella parvula和Corynebacterium sp..采用GC-MS分析出口气样,结果表明丙酮酸(CH3COCOOH)为生物降解苯酚的中间产物,并推测了苯酚生物降解的可能途径.The performance of a bio-trickling filter （BTF） for treatment of phenol, a model pollutant, was presented. Influences of factors on phenol removal efficiency were studied. The BTF exhibited a high removal efficiency for phenol. The experimental results showed that the phenol efficiency reached 99.5% and kept 98% in the long-term run. The optimal residence time, pH value and spray density were 20.6 s, 7.0 and 1.67 m3· （m2·h）-t, respectively. The microbial community structures in the bio-trickling filter for phenol removal were assessed by PCR-DGGE. Based on the 16S rDNA sequence data,results showed that the predominant bacteria for degradation of phenol were Polaromonas sp. , Acinetobacter sp. , Acidovorax sp. , Veillonella parvula and Corynebacterium sp.. GC-MS was used to detect component of BTF＇s outlet gases and pyruvie acid（CH3COCOOH） was found as one kind of intermediates of phenol degradation. Then one possible biodegradation pathway of phenol was inferred.

关 键 词：苯酚 生物滴滤塔 PCR—DGGE GC—MS 机制 

分 类 号：X701[环境科学与工程—环境工程]