热敏灸减少脑缺血再灌注损伤模型大鼠大脑皮质细胞色素C与Bax蛋白的表达  

Heat- sensitive moxibustion reduces cortical expressions of cytochrome C and Bax protein in rats with cerebral ischemia- reperfusion injury

作　　者：肖爱娇[1] 康明非[2] 陈日新[2] 熊浩仲[1] 

机构地区：[1]江西中医药大学基础医学院,江西南昌330004 [2]江西中医药大学附属医院,江西南昌330006

出　　处：《时珍国医国药》2014年第1期240-243,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81060305);江西省卫生厅中医药科研计划课题(No.2012A004)

摘　　要：摘要：目的观察热敏灸对大脑皮质细胞色素C（CytC）与Bax蛋白表达的影响，阐明热敏灸减轻脑缺血再灌注损伤的作用机制。方法雄性SD大鼠42只，分为正常组（6只）、假手术组（6只）、模型组（10只）、艾灸组（10只）和热敏灸组（10只）。采用线栓法闭塞大脑中动脉2h后进行再灌注，制备局灶性脑缺血再灌注损伤大鼠模型。采用神经功能缺失评分观察大鼠的行为学表现；HE染色观察大鼠脑组织形态结构；Westernblot技术检测大鼠大脑皮质CytC与Bax蛋白的表达。结果假手术组大鼠神经功能缺失评分为0分，其余组大鼠神经功能缺失评分为1～3分。与假手术组比较，模型组大鼠左侧脑组织可见神经细胞大量坏死脱落、胞质呈空泡变性、疏松、胞核浓缩深染；大脑皮质CytC与Bax蛋白表达量明显增加；与模型组比较，热敏灸组大鼠脑组织排列较致密、整齐；大脑皮质CytC与Bax蛋白表达量明显减少。结论热敏灸具有减轻大鼠脑缺血再灌注损伤的作用，降低大脑皮质CytC与Bax蛋白的表达可能是其作用机理之一。Objective To observe the effect of heat - sensitive moxibustion on cortical expressions of cytochrome C and Bax pro- tein of rats with cerebral ischemia- reperfusion injury. Methods Forty- two male SD rats were randomly divided into five groups, normal group （ n = 6）, sham group （ n = 6）, model group （ n = 10）, moxibustion group （ n = 10） and heat - sensitive moxi- bustion group（ n = 10）. Focal cerebral ischemia -reperfusion injury model was induced for 2h by middle cerebral artery occlusion （MCAO） followed by reperfusion. Neurological deficit scores were evaluated to observe behaviors. HE staining was used to exam- ine brain morphological structure, and Western blot was to detect the expressions of cytochrome C and Bax protein in the cerebral cortex. Results The neurological deficit scores were 1 -3 score（s） after 2h for MCAO followed by reperfusion while the score was 0 after sham operation, which suggested that rats model of focal cerebral ischemia - reperfusion injury were successful. Compared with sham group, there were a large number of nerve cells necrosis, cytoplasm osteoporosis and condensed stained nuclei in the brain of model group,and the cortical expressions of cytochrome C and Bax protein were significantly increased. Compared with model group, fewer nerve cells experienced necrosis and cortical expressions of cytochrome C and Bax protein were reduced in heat - sensitive moxibustion group. Conclusion Heat - sensitive moxibustion attenuates focal cerebral ischemia - reperfusion inju- ry, which might be related to reducing expressions of cytochrome C and Bax protein.

关 键 词：关键词 热敏灸 脑缺血再灌注损伤 细胞色素C BAX 

分 类 号：R23[医药卫生—中医学]