水稻叶绿体基因组中一个编码psbL和psbJ基因cDNA的克隆与分析  

Cloning and Analysis of a cDNA Encoding psbL and psbJ Gene in Rice Chloroplast Genome

作　　者：顾克余[1] 罗林广[1] 苏昌潮[1] 翟虎渠[1] 

机构地区：[1]南京农业大学作物遗传与特异种质创新教育部重点实验室,南京210095

出　　处：《Acta Botanica Sinica》2001年第2期210-212,共3页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家重点基础研究专项经费资助! (G19980 10 10 0 )&&

摘　　要：A 505 bp cDNA was cloned from the leaves of rice (Oryza sativa L.) Shanyou 63 combination. DNA sequence analysis showed that it is a part of rice chloroplast genome. Its homology comparison with those known in GenBank found that it encodes 38 amino acid peptide deduced from psbL gene and 40 amino acid peptide deduced from psbJ gene in rice chloroplast PSⅡ. Northern hybridization showed that the cDNA was differentially displayed in hybrid F 1 and its parental lines.A 505 bp cDNA was cloned from the leaves of rice (Oryza sativa L.) Shanyou 63 combination. DNA sequence analysis showed that it is a part of rice chloroplast genome. Its homology comparison with those known in GenBank found that it encodes 38 amino acid peptide deduced from psbL gene and 40 amino acid peptide deduced from psbJ gene in rice chloroplast PSⅡ. Northern hybridization showed that the cDNA was differentially displayed in hybrid F 1 and its parental lines.

关 键 词：水稻 psBL psBJ CDNA克隆 基因表达 差异显示 叶 

分 类 号：Q943.2[生物学—植物学]