抗氧化剂对铀矿尘致巨噬细胞损伤保护作用  

Protective effect of four antioxidants on human macrophage injury caused by uranium dust

作　　者：曹芳[1] 朱茂祥[2] 杨陟华[2] 潘秀颉[2] 贺性鹏[1] 

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001 [2]军事医学科学院放射与辐射医学研究所

出　　处：《中国公共卫生》2014年第2期239-241,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30670634)

摘　　要：目的探讨N-乙酰半胱胺酸(NAC)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、甘露醇4种抗氧化剂对铀矿尘致巨噬细胞损伤的保护作用。方法采用四甲基偶氮唑蓝(MTT)法、荧光显微镜及化学/荧光发光仪检测不同浓度铀矿尘处理巨噬细胞后细胞存活率、细胞内活性氧(ROS)以及还原型谷胱甘肽(GSH)水平变化;采用annexin V/PI双染法检测4种抗氧化剂对铀矿尘致巨噬细胞凋亡的保护作用。结果与对照组比较,铀矿尘染毒组巨噬细胞存活率下降,胞内ROS含量上升,GSH水平下降,呈时间-剂量效应关系(P<0.05);抗氧化剂NAC、CAT、SOD、甘露醇孵育后,与对照组比较,NAC和甘露醇组巨噬细胞存活率[分别为(89.00±0.95)%、(85.87±2.20)%]明显升高,ROS含量[分别为(3.87±0.41)、(6.47±0.99)]下降,GSH水平[分别为(22.81±2.90)、(21.20±2.85)]升高(P<0.05)。结论 NAC和甘露醇对铀矿尘致巨噬细胞损伤具有保护作用并明显优于CAT与SOD。Objective To investigate the protective effects of four antioxidants （ N-acetyl cysteine [ NAC ], catalase [ CAT] ,superoxide dismutase[ SOD], and mannitol）on oxidative injury of human macrophages induced by uraniumdust. Methods The cell viability, intracellular reactive oxygen species （ROS）and glutathione （GSH）were determined with methyl thiazolyl tetrazolium （MTT）assay, fluorescence microscopy and chemical/fluorescence emission, respectively. The protective effects of the four antioxidants on uranium dust-induced oxidative injury were evaluated with a combination of MTT and annexin V-FITC/PI staining methods. Results Uranium dust significantly reduced cell via- bility in a concentration- and time-dependent manner（P 〈 0. 05 for all）. Uranium dust-induced apoptosis was character- ized by the increare of ROS and the decrease of GSH. After pretreated with NAC, CAT, SOD, and mannitol, the survival rate of human macrophages （ 89.00 ± 0. 95% and 85.87 ± 2. 20% ） was apparently increased, the concentration of ROS （3.87 ±0.41 and 6.47 ±0. 99） was decreased and the level of GSH（22. 81 ±2. 90 and 21.20 ±2. 85） was increased sig- nificantly compared to the cells without NAC or mannitol pretreatment. Conclusion NAC and mannitol are more effec- tive in protecting human macrophages against oxidative injury induced by uranium dust than SOD and CAT.

关 键 词：铀矿尘 巨噬细胞 凋亡 抗氧化剂 

分 类 号：R995[医药卫生—毒理学]