四君子汤对脾虚证大鼠胃黏膜上皮细胞凋亡及Caspase-3表达的影响  

Influence of Sijunzi Decoction on Gastric PCD and Caspase-3 Expression in Spleen Deficient Rats

作　　者：赵盛云[1,2] 王垂杰[3] 

机构地区：[1]辽宁中医药大学研究生学院,沈阳110847 [2]中国医科大学附属第一医院中医科,沈阳110001 [3]辽宁中医药大学附属医院消化科,沈阳110032

出　　处：《中国医科大学学报》2014年第1期31-33,共3页Journal of China Medical University

基　　金：国家自然科学基金(81173154)

摘　　要：目的探讨脾虚证大鼠模型胃黏膜上皮细胞凋亡变化情况及四君子汤对其改变的影响。方法 102只SD大鼠随机分为正常对照组(n=24)、长期造模组(n=26)、自然恢复组(n=26)和四君子汤组(n=26)。正常对照组常规饲养7周,前4周给予生理盐水隔日1次灌胃,后3周每日1次灌胃(1.5 mL/100 g)。其余3组均采用复合因素造模法复制脾虚证大鼠模型,造模4周结束后,长期造模组继续造模3周,自然恢复组常规喂养3周,四君子汤组给予四君子汤每日1次灌胃3周(1.5 mL/100 g)。用TUNEL方法检测各组大鼠胃黏膜上皮细胞的凋亡变化,用ABC免疫组化方法检测Caspase-3调控蛋白的变化。结果四君子汤组大鼠胃黏膜Caspase-3含量及上皮细胞凋亡程度均降低,与长期造模组及自然恢复组比较有显著差异(P<0.05),但仍高于正常对照组(P<0.05)。结论四君子汤可以减少损伤的胃黏膜细胞的凋亡,减少胃黏膜疾病的发生。Objective To discuss gastric cell death（PCD） changes in the spleen deficient rat models, and the influence of Chinese medicine Sijun- zi decoction on the changes. Methods A total of 102 SD rats were randomly divided into the normal control group （n =24）, the long-term model group （n =26）, the natural recovery group （n =26） and the Sijunzi decoction group （n =26）. Rats in the normal control group were raised in the rou- tine way for 7 weeks ; they were given normal saline 1.5 mL/l00 g by gavage once every other day during the f＇wst 4 weeks, and then everyday in the last 3 weeks. Spleen deficiency rat model was made by composite factors in the other three groups. After 4-week modeling, the rats in the long-term group were modeled for another 3 weeks. Rats in the natural recovery group were raised in the routine way for 3 weeks. Rats in the Sijunzi decoction group were given Sijunzi decoction 1.5 mIJl00 g by gavage once everyday for 3 weeks. Tunnel assay was used to observe the prograrmned ceil death level and immunohistochemistry was used to evaluate Caspase-3 expression in rat stomach tissues. Results The Caspase-3 content and epithelial cell death level in Sijunzi decoction group decreased （P 〈 0.05）. Compared with those in the long-term model group and natural recovery group, the difference was significant （P 〈 0.05 ）. However, the levels are higher than those of normal control group （P 〈 0.05 ）. Conclusion Sijunzi decoction can reduce the chronic stomach diseases by improving the gastric mucosal barrier function.

关 键 词：脾虚证 四君子汤 胃黏膜屏障 细胞凋亡 Caspase-3 

分 类 号：R2[医药卫生—中医学]