高脂饲养胰岛素抵抗大鼠血清Apelin-36水平的变化  

Changes of Plasma Apelin-36 Level in High-fat-diet Fed Rats with Insulin Resistance

作　　者：陈薇[1] 赵晓娟[1] 王楠楠[1] 刘项楠[2] 

机构地区：[1]中国医科大学附属第一医院内分泌科,沈阳110001 [2]辽宁省人民医院血液科,沈阳110016

出　　处：《中国医科大学学报》2014年第1期52-54,共3页Journal of China Medical University

基　　金：辽宁省科学技术计划(2009225010-10)

摘　　要：目的探讨肥胖的胰岛素抵抗大鼠血清Apelin-36水平的变化。方法分别用基础饲料、高脂饲料制备正常对照组(NC组)及胰岛素抵抗组(HF组)大鼠模型,10周后2组各取5只行高胰岛素—正葡萄糖钳夹实验评估模型。测定体质量、附睾脂肪质量指数、空腹尾尖血糖,放免法检测血清胰岛素(INS)水平,ELISA法测定血清Apelin-36、游离脂肪酸(FFA)水平。结果 (1)HF组较NC组体质量明显升高(P<0.01)、附睾脂肪质量指数明显升高(P<0.01);(2)2组大鼠基础血糖比较无统计学差异(P>0.05),HF组血清基础胰岛素、Apelin-36及游离脂肪酸较NC组明显升高(P<0.01);(3)血清Apelin-36与基础胰岛素、游离脂肪酸、附睾脂肪质量指数呈正相关(r分别为0.452、0.637、0.756,P均<0.05)。结论血浆Apelin-36水平在胰岛素抵抗大鼠组升高,推测在胰岛素抵抗状态下Apelin已代偿性分泌增加以改善胰岛素抵抗。Objective To study whether there are changes in the plasma Apehn-36 level in obese rats with insulin resistance. Methods By feed- ing rats with normal diet and high-fat diet, normal control group and insulin resistant group models were induced. After 10 weeks, 5 rat models in each group were evaluated by the hyperinsulinemic-euglycemic clamp technique. The levels of weight, epididymal fat mass index （BMI）, plasma fasting plasmaglucose （ FPG）, insulin, Apehn - 36 and free fatty acid （FFA） were detected, compared and analyzed. Results （ 1 ） Compared with the control group, the weight and epididymal fat mass index （BMI） increased significantly in the insulin resistant models. （2） There was no signifi- cant difference in basic blood glucose of the two groups of rats （P 〉 0.05 ） ; univariate analysis revealed that serum insulin, Apelin-36 and FFA in the insulin resistant group increased significantly compared with the control group （P 〈 0.01 ）. （3） Pearson relation analysis showed that the level of Ape- lin- 36 was positively correlated with basic quality of insulin, FFA and epididymal fat mass index （BMI） （r1 = 0.452, P 〈 0.05 ; r2 = 0.637, P 〈 0.01 ; r3 = 0.756, P 〈 0.01 ）. Conclusion The plasma Apelin-36 level is higher in the insulin resistant group than in the control group, which im- phes that maybe in the state of insulin resistance, Apelin has compensatory secretion to improve insulin resistance.

关 键 词：胰岛素抵抗 高胰岛素一正葡萄糖钳夹试验 APELIN 

分 类 号：R587.1[医药卫生—内分泌]