体外受精—胚胎移植中不同授精浓度对胚胎质量及妊娠结局的影响  

Effect of Different Sperm Insemination Concentrations on Embryo Quality and Pregnancy Outcome in IVF-ET

作　　者：杨大磊[1] 潘伯臣[1] 何丽霞[1] 王秀霞[1] 王天任[1] 李正涛[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科辅助生殖中心,沈阳110004

出　　处：《中国医科大学学报》2014年第1期67-70,共4页Journal of China Medical University

基　　金：辽宁省教育厅高校科研计划(LT2010106)

摘　　要：目的通过比较不同授精浓度下胚胎发育及妊娠结局，探讨体外受精-胚胎移植（WF-ET）中较为合适的授精浓度。方法对因输卵管因素接受1VF-ET的夫妇进行随机对照试验，根据不同授精浓度随机分为3组：A组为（0．1～0．5）×106／mL，B组为（0．5-1．0）×10^6／mL，C组为（1．0-1．5）×106／mL。比较3组间正常受精率、多精受精率、卵裂率、优质胚胎率、临床妊娠率、着床率及流产率。结果3组的正常受精率、卵裂率、优质胚胎率及流产率均无统计学差异（P〉0．05），但B组和C组的多精受精率（分别为14．9％和13．2％）明显高于A组（8．6％）（P〈0．05），而且C组的妊娠率及种植率（50．0％，29．5％）明显高于B组（41．4％，20．4％）和A组（38．6％-18．8％）（P〈0．05）。结论IVF．ET治疗中，增加授精滴中精子浓度可导致卵子多精受精增加，但在一定范围内增加精子浓度可能改善妊娠结局。Objective To explore the optimal sperm insemination concentration for the in vitro fertilization-embryo transfer （IVF-ET） procedure through comparison of the outcomes of IVF-ET with different sperm insemination concentrations. Methods A randomized controlled trial was car- fled out in 210 couples who received IVF-ET treatment for tubal factor infertility. Couples were randomly divided into three groups according to the insemination concentrations： group A with concentration （0.1-0.5） x 106/mL, group B （0.5-1.0） x 106/mL, and group C （ 1.0-1.5 ） x 106/mL. Normal fertilization rate, polyspermy rate, cleavage rate, high-quality embryo rate, pregnancy rate, implantation rate and abortion rate were compared be- tween the three groups. Results No significant difference was found in normal fertilization rate, cleavage rate, high-quality embryo rate and abortion rate between the three groups （P 〉 0.05）. However, the polyspermy rates of group B （ 14.9% ） and group C （ 13.2% ） were significantly higher than that of group A （ 8.6% ） （P 〈 0.05 ）. In addition, the pregnancy rate （ 50.0% ） and implantation rate （29.5%） of group C were significantly higher than those of group B （41.4% and 20.4% ） and group A （ 38.6% and 18.8% ）, respectively （P 〈 0.05 ）. Conclusion Increasing the sperm concen- tration of insemination in the ctdture droplet in IVF-ET treatment may increase the risk of polyspermy fertilization. However, increasing the sperm in- semination concentration within a certain scope may improve the treatment outcome.

关 键 词：体外受精-胚胎移植 精子浓度 授精 多精受精 妊娠率 

分 类 号：R321.33[医药卫生—人体解剖和组织胚胎学]