注射用姜黄素温敏凝胶体内外抗肿瘤实验研究  

Study on Anti-tumor in vivo and in vitro Experiments of Curcumin Thermosensitive hydrogels for Injection

作　　者：高萌[1] 蒋妮 徐红[3] 张成鸿[3] 关欣[1] 李磊[1] 李镇[1] 田燕[1] 

机构地区：[1]大连医科大学药学院,辽宁大连116044 [2]大连儿童医院药剂科,辽宁大连116001 [3]大连医科大学基础医学院,辽宁大连116044

出　　处：《中国药房》2014年第7期605-608,共4页China Pharmacy

摘　　要：目的:研究注射用姜黄素温敏凝胶体外对小鼠腹水型肝癌高淋巴道转移细胞株(HCA-F)增殖的抑制作用和体内对移植瘤模型小鼠的保护作用。方法:培养HCA-F细胞株,MTT法测定10、25、50、75、100μg/ml注射用姜黄素温敏凝胶、氟尿嘧啶、姜黄素混悬液对HCA-F细胞增殖的抑制作用。抽取腹水型肝癌模型小鼠腹水接种于小鼠腋下以复制小鼠移植瘤模型。50只小鼠随机均分为模型(等容生理盐水)组、空白温敏凝胶(ETH,0.05 ml/cm3))组、无水乙醇(0.05 ml/cm3)组、姜黄素混悬液(0.05 ml/cm3)组、注射用姜黄素温敏凝胶(0.05 ml/cm3)组,瘤内注射给药,每隔5 d1次,连续2次。测定小鼠瘤体增长体积、瘤质量、抑瘤率,HE染色检测肿瘤细胞凋亡情况。结果:培养细胞48 h时,25、50、75、100μg/ml注射用姜黄素温敏凝胶对HCA-F细胞增殖有明显抑制作用(P<0.05);培养细胞72 h时,10、25、50、75、100μg/ml注射用姜黄素温敏凝胶对HCA-F细胞增殖有明显抑制作用(P<0.05)。与模型组比较,注射用姜黄素温敏凝胶组小鼠瘤体体积增长程度明显减小,瘤质量明显减轻,抑瘤率明显增加,差异有统计学意义(P<0.05);注射用姜黄素温敏凝胶组小鼠肿瘤细胞凋亡情况明显改善。结论:注射用姜黄素温敏凝胶在体外对HCA-F增殖具有一定的抑制作用,且体内效果与无水乙醇相当,但小鼠的顺应性较无水乙醇好。OBJECTIVE： To investigate the inhibitory effects of Curcumin thermosensitive hydrogels for injection （CTH） on ascitic hepatocarcinoma cell strain with high metastasis potential in lymphatic system （HCA-F） and protective effects of it on solid tumors model mice. METHODS： HCA-F cell strain were cultured, and inhibitory effects of 10, 25, 50, 75 and 100 }.tg/ml CTH on the proliferation of HCA-F cell were determined by MTT. Mice solid tumors model was induced by vaccinated with ascites of ascitic hepatocarcin0ma model mice into axillary fossa. 50 Kunming mice were divided into model group （constant volume of normal saline）, blank thermosensitive gel group （ETH 0.05 ml/cm3）, absolute ethyl alcohol group （absolute ethyl alcohol 0.05 ml/cm3）, curcumin group （0.05 ml/cm3） and CTH group （0.05 ml/m3）. They were given relevant medicines intratumorally every 5 days for consecutive 2 times. The increase of tumor volume, tumor weight and inhibitory rate were determined, and the apoptosis of tumor cells was determined by HE staining. RESULTS：25, 50, 75 and 100μg/ml CTH significantly inhibited the proliferation of HCA-F after cultured for 48 h. 10, 25, 50, 75 and 100 μg/ml CTH significantly inhibited the proliferation of HCA-F after cultured for 72 h. Compared with model group, the growth rate of tumor volume as well as tumor weight were decreased significantly in CTH group, while inhibitory rate increased significantly; there was statistical significance （P〈0.05）. The apoptosis of tumor cells in CTH groups was improved significantly. CONCLUSIONS： CTH could effectively inhibit the proliferation of HCA-F in vitro, which is similar to the effect of absolute ethyl alcohol in vivo. The mice is more adaptable to CTH than absolute ethyl alcohol.

关 键 词：注射用姜黄素温敏凝胶 腹水型肝癌高淋巴道转移细胞株 小鼠肝癌移植瘤 

分 类 号：R284.62[医药卫生—中药学] R284.2[医药卫生—中医学]