HPLC法测定石见穿中丹酚酸B的含量  

Content Determination of Salvianolic Acid B in Salvia chinensis by HPLC

作　　者：高俊峰[1] 丁玲[1] 张鹏[1] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《中国药房》2014年第7期639-640,共2页China Pharmacy

基　　金：北京市科技专项(No.Z121107002412021)

摘　　要：目的：建立测定石见穿中丹酚酸B含量的方法。方法：采用高效液相色谱法。色谱柱为Thermo C18（150mm×4．6mm，5μm），流动相为乙腈-0．1％甲酸水溶液（21：79，V／V），流速为1．0ml／min，检测波长为286nm，柱温为23℃。结果：丹酚酸B的进样量在0．153-3．060gg范围内与其峰面积积分值呈良好线性关系（r=0．9998）；精密度、稳定性、重复性试验的RSD〈1％；平均加样回收率为97．70％，RSD=0．56％（n=6）。结论：该方法操作简便、准确可靠、重复性好，可作为石见穿药材的质量控制方法。OBJECTIVE： To establish the method for the content determination of salvianolic acid B in Salvia chinensis. METHODS： HPLC method was adopted. The determination was performed on Thermo C18（150 mm×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-0.1% formic acid solution（21 ： 79, V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 286 nm,and the column temperature was 23 ℃. RESULTS：The linear range of salvianolic acid B were 0.153-3.060 μg（r=0.999 8） with an average recovery of 97.70% （RSD=0.56%, n=6）; RSD of precision, stability and reproducibility tests were lower than 1%. CONCLUSIONS： The method is simple, accurate, reliable, repeatable and can be used for quality control of S. chinensis.

关 键 词：石见穿 丹酚酸B 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]