HPLC-ELSD法测定妇安栓剂中黄芪甲苷的含量  

Content Determination of Astragaloside in Fuan Suppositories by HPLC-ELSD

作　　者：宋敏[1] 

机构地区：[1]资阳市第一人民医院药剂科,四川资阳641300

出　　处：《中国药房》2014年第7期647-648,共2页China Pharmacy

摘　　要：目的：建立测定妇安栓剂中黄芪甲苷含量的方法。方法：采用高效液相色谱．蒸发光散射检测器法。色谱柱为Phenomenex C18（250mm×4．6mm，5μm），流动相为乙腈-水（32：68，V／V），流速为0．8ml／min，柱温为30℃，蒸发温度为100cc，氮气流速为2．0L／min。结果：黄芪甲苷进样量在2．65－26．50pg范围内的对数值与峰面积积分值的对数值呈良好的线性关系（r=0．9999）；精密度、稳定性、重复性试验的RSD〈3％；平均加样回收率为99．38％，RSD=1．60％（n=9）。结论：该方法简便、灵敏、重复性好，可用于妇安栓剂的质量控制。OBJECTIVE： To establish a method for the content determination of astragaloside in Fuan suppositories. METHODS： HPLC-ELSD was adopted. The determination was performed on Phenomenex C,8（250 mm×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-water （32 ： 68, V/V） at the flow rate of 0.8 ml/min. The column temperature was set at 30 ℃, and evaporation temperature was 100 ℃. The flow rate of nitrogen was 2.0 L/min. RESULTS： The linear range of astragaloside was 2.65-26.50 μg（r=0.999 9） ; RSDs of precision, stability and reproducibility tests were lower than 3% ; average recovery was 99.38% （RSD= 1.60%, n=9）. CONCLUSIONS： The method is simple, sensitive and reproducibility. It can be used for the quality control for Fuan suppositories. KEYWORDS Fuan suppositories; Astragaloside; HPLC-ELSD; Content determination

关 键 词：妇安栓剂 黄芪甲苷 高效液相色谱-蒸发光散射检测器法 含量测定 

分 类 号：R283.623[医药卫生—中药学] R927.2[医药卫生—中医学]