检测马疱疹病毒4型抗体间接ELISA方法的建立及应用  

Establishment and Application of ELISA for Detection of Equine Herpesviruses Type 4 Antibodies

作　　者：田志革[1,2] 郭巍[1] 赵世华[1] 潘佳亮[2] 冉多良[3] 相文华[1] 曲娟娟[2] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [2]东北农业大学生命科学学院,黑龙江哈尔滨150030 [3]新疆农业大学动物医学学院,新疆乌鲁木齐830052

出　　处：《中国畜牧兽医》2014年第1期11-14,共4页China Animal Husbandry & Veterinary Medicine

基　　金："中央级公益性科研院所基本科研业务费专项"(0302012011);"十二五"农村领域国家科技计划课题;马匹疫病诊断与综合防控技术研究与示范(2012BAD46B01-02)

摘　　要：为建立检测马疱疹病毒4型(EHV4)抗体的间接ELISA方法,本研究以原核表达的具有型特异性抗原表位的gG蛋白为检测抗原,经条件优化,成功建立检测EHV4抗体的间接ELISA方法,对该方法进行特异性及重复性试验。结果表明,重组4型gG蛋白仅与EHV4阳性血清发生反应,不与马疱疹病毒1型(EHV1)阳性血清发生反应;组内及组间变异系数均小于10%。采用建立的方法与进口试剂盒对不同省份送检的马血清进行检测比较,两者阳性和阴性符合率均在90%以上。本研究建立的间接ELISA方法为检测EHV4抗体及流行病学调查奠定基础。In order to establish an indirect ELISA method for detection of equine herpesviruses type 4 （EHV4） antibody, the glycoprotein G （gG） protein with specific epitope worked as a detection antigen. After optimizing conditions, the indirect ELISA method was developed successfully，specificity and repeatability tests were determined. The result was that the EHV4 gG only reacted with antibodies against EHV4;but not with antibodies against EHV1; both the intro-batch and inter-batch variation coefficiencies were lower than 10%.Concordance of the indirect ELISA relative to commercial EHV1/4 antibody kit was above 90%.The results indicated that the indirect ELISA method could be used for the detection and epidemiological surveys of EHV4 infection.

关 键 词：检测 马疱疹病毒4型 gG蛋白 间接ELISA 

分 类 号：S852.4[农业科学—基础兽医学]