羧甲基葡聚糖注射剂药效学研究  

Study on the Pharmacological Effects of Carboxymethyl Glucan(CMG)Injection

作　　者：孙英峰[1,2] 王英珍[2] 池晶晶[2] 王东[2] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]天津市畜牧兽医研究所,天津300381

出　　处：《中国畜牧兽医》2014年第1期133-136,共4页China Animal Husbandry & Veterinary Medicine

基　　金：天津市应用基础及前沿技术研究计划(12JCYBJC20100)

摘　　要：试验旨在研究羧甲基葡聚糖(CMG)注射剂药效,从而为临床动物疾病防制提供试验依据。试验选取小鼠作为试验动物,进行急性毒性试验及体内抗金黄色葡萄球菌、无乳链球菌及大肠杆菌感染试验。另外,采用MTT方法测定了CMG对奶牛淋巴细胞转化率影响,并对CMG注射剂进行了奶牛临床初步应用试验。结果表明,小白鼠对CMG最大耐受量在240mg/只以上,且在4mg/只浓度下对金黄色葡萄球菌、无乳链球菌及大肠杆菌感染的保护率分别为60%、62.5%、87.5%;CMG能明显提高奶牛淋巴细胞转化率,临床应用试验组比空白对照组隐性乳腺炎发病率下降26.8%。本试验结果表明,CMG注射液可作为潜在新型兽用免疫增强剂在动物临床应用。This assay was aimed to study the pharmacological effects of carboxymethyl glucan （CMG） injection，so as to provide experimental bases for clinical diseases use. The mice were used to study acute toxicity assay, the ability against Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli. In addition, lymphocyte transformation rates of dairy cows were determined by MTT method, and the clinical application of CMG injection was also carried out. The results indicated that the maximum tolerance dose of mice to CMG was above 240 mg per mouse, and in the 4 mg per mouse, the rates of protection against Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli were 60%, 62.5%, 87.5%. CMG could apparently increase the lymphocyte transformation rate of dairy cows. In clinical application, the mastitis incidence of experimental group decreased by 26.8% compared with the blank control. All the results above showed that CMG injection could be used as a potential new immunopotentiator for clinical application in animals.

关 键 词：羧甲基葡聚糖 注射剂 抗感染 免疫增强 临床应用 

分 类 号：Q538[生物学—生物化学]