京海黄鸡柔嫩艾美尔球虫抗性指标的研究  

Study on Resistant Indexes of Eimeria tenella for the Jinghai Yellow Chicken

作　　者：孙明明[1,2] 林雨鑫[1,2] 戴国俊[1,2] 孙大辉[1,2] 尉浩华 张跟喜[1,2] 谢恺舟[1,2] 王成丽[1,3] 施会强[4] 王金玉[1,2] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [3]江苏农牧科技职业学院,江苏泰州225300 [4]江苏京海禽业集团有限公司,江苏海门226103

出　　处：《中国畜牧兽医》2014年第1期199-203,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家肉鸡产业技术体系项目(nyctx-42-G1-05);江苏省高校自然科学研究重大项目(11KJA230001);江苏高校优势学科建设工程项目(PAPD);国家及江苏省大学生实践创新训练项目(201211117035;2012JSSPITP1347);江苏省产学研创新资金前瞻性联合研究项目(BY2013063-06)

摘　　要：为进一步筛选有效的鸡球虫病抗性评价指标，并为后续鸡球虫抗性评价体系的建立及鸡球虫抗病育种提供依据，试验将京海黄鸡分为感染和非感染对照2个组，感染组每只雏鸡灌饲2.5×104个柔嫩艾美尔球虫卵囊，用酶联免疫检测法测定10个球虫抗性血液指标。结果显示，①感染后第3天感染组和非感染对照组的临床表现和各项指标差异均不明显，表明感染后这一阶段无法用现有指标检测到显著变化；②感染后3～5 d感染组鸡体增重为负，且感染组和非感染对照组相比差异极显著（P＜0.01），表明感染后3～5 d的变化最为剧烈；③感染后8 d IFN-γ浓度显著低于非感染对照组（P＜0.05）；丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽—过氧化物酶（GSH-Px）、过氧化氢酶（CAT）浓度均有不同程度提高，且感染组与非感染对照组SOD浓度差异显著（P＜0.05），其余3个指标均差异极显著（P＜0.01）；④白细胞介素2（IL-2）、IL-16、IL-17、一氧化氮（NO）、β-胡萝卜素（β-C）浓度感染组和非感染对照组相比差异均不显著（P＞0.05）。结果表明，感染后3～5 d内的体增重，CAT、GSH-Px、MDA、SOD 4种抗氧化酶及IFN-γ细胞因子在血浆中的浓度可作为鸡球虫抗性评价指标。The purpose of the research was to make preparations for establishing the disease resistance evaluation system as well as laying the basis for disease-resistance breeding of the Jinghai Yellow chicken. Chicken were divided into infection and control groups, the infection group was orally inoculated with Eimeria tenella oocysts （2.5×104 oocysts per bird）. 10 resistance associated blood indexes were measured through the method of ELISA. The results showed，the difference of indexes 3rd day after inoculation was not significant （P＞0.05） which suggested that it was hard to detect any significant change within these indexes in this period of infection;3rd to 5th day after inoculation, the body weight gain was extremly significantly higher than that of control group （P＜0.01）;the concentration of IFN-γ 8th day after inoculation was significantly lower than that of the control group, the concentration of SOD 8th day after inoculation was significantly higher than that of the control group （P＜0.05）,and the concentrations of MDA,GSH-Px,CAT were extremely significantly higher than those of the control group （P＜0.01）；the concentrations of IL-2,IL-16,IL-17,NO and β-carotene had no significant difference with control group （P＞0.05）. 3rd to 5th day after inoculation，the body weight gain, the concentrations of the 4 antioxidant enzymes （CAT, GSH-Px, MDA, SOD） and IFN-γ could be taken as the candidate indexes for the disease resistance evaluation system of the Jinghai Yellow chicken.

关 键 词：京海黄鸡 柔嫩艾美耳球虫 抗球虫指标 

分 类 号：S852.7[农业科学—基础兽医学]