兰州地区部分奶牛场子宫内膜炎病原菌分离鉴定及抗生素耐药性研究  

Isolation and Identification of Endometritis Pathogens in Dairy Farms Located in Lanzhou City and its Antibiotic Resistance Analysis

作　　者：李宏胜[1] 杨峰[1] 王旭荣[1] 陈炅然[1] 胡广胜[1] 罗金印[1] 李新圃[1] 苗小楼[1] 王玲[1] 朱新荣 刘学成 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省新兽药工程重点实验室,甘肃兰州730050 [2]甘肃荷斯坦奶牛繁育示范中心,甘肃兰州730086

出　　处：《中国畜牧兽医》2014年第1期222-226,共5页China Animal Husbandry & Veterinary Medicine

基　　金：兰州市科技计划(2011-1-70);甘肃省国际合作项目(1204WCGA019)

摘　　要：为了探明兰州地区奶牛子宫内膜炎病原菌区系分布及抗生素耐药情况，指导临床合理用药。笔者对从兰州地区3个规模化奶牛场采集的47份奶牛子宫内膜炎样品进行了细菌分离鉴定，同时对分离鉴定出的4种主要病原菌采用纸片扩散法（K-B法）进行了抗生素耐药性检测。病原菌区系分布结果表明，引起兰州地区奶牛子宫内膜炎的病原菌主要为化脓隐秘杆菌（37.1%）、大肠杆菌（24.2%）、屎肠球菌（22.6%）和无乳链球菌（16.1%）。抗生素耐药性结果表明，4种主要病原菌对大部分抗生素均产生了不同程度的耐药性，尤其是对青霉素、复方新诺明、奥复星和杆菌肽耐药性最为严重，其耐药率为60%～100%。对4种病原菌均较敏感的药物主要有头孢他啶、先锋霉素V和氟苯尼考，其敏感度达100%。This study was planned to investigate the distribution and antibiotic resistance of pathogens involve in cow endometritis，which will be helpful to guide clinical rational drug use.All 47 cow endometritis samples collected from three large-scale farms located in Lanzhou were used to isolate and identify pathogens. Besides，K-B paper disk method was performed to measure the antibiotic resistance of the four isolates. The results demonstrated that Arcanobacterium pyogenes （37.1%），Escherichia coli （24.2%），Enterococcus faecium （22.6%） and Streptococcus agalactiae （16.1%） were the predominent agent causing cow endometritis in Lanzhou.In addition，the four pathogens were resistant to penicillin，sulfamethoxazole compound，ofoxacin and bacitracin，and the drug resistance rates reached 60% to 100%. However，These pathogens were sensitive to ceftazidime，cephalothin V and florfenicol，and the antibiotic sensitive rates were 100%.

关 键 词：奶牛子宫内膜炎 病原菌 分离鉴定 抗生素耐药性 

分 类 号：S852.61[农业科学—基础兽医学]