老年COPD患者CD4^+ Foxp3^+ T细胞、PCT、NF-κB的检测及临床应用  

The change of CD4^+Foxp3^+regulatory T cells,procalcitonin,nuclear factor kappa-B in elderly COPD patients and their clinical application

作　　者：汤小芳[1] 陈旭娇[1] 柴栖晨[1] 程磊[1] 

机构地区：[1]浙江医院老年病综合示范病房,杭州市310013

出　　处：《中华全科医学》2014年第2期241-243,F0003,共4页Chinese Journal of General Practice

摘　　要：目的观察老年慢性阻塞性肺病(COPD)急性加重期、稳定期患者和健康老人CD4+Foxp3+调节性T细胞(Treg细胞)比例、降钙素原(PCT)以及痰巨噬细胞中核因子-B(NF-κB)p65亚单位表达的变化,为临床诊疗提供参考。方法 42例COPD急性加重期老年患者为观察组1,39例COPD稳定期老年患者为观察组2,35例健康老人为对照组。检测3组患者Treg细胞比例、PCT水平以及NF-κB p65百分比。结果①3组之间外周血Treg细胞比例差异有统计学意义(P<0.05);②观察组1与观察组2、对照组之间PCT水平均差异显著(P<0.05),观察组2与对照组之间PCT水平差异无统计学意义(P>0.05);③3组之间痰巨噬细胞NF-κB P65差异有统计学意义(P<0.05)。结论炎症机制及免疫机制均在老年COPD患者中发挥了重要作用。监测CD4+Foxp3+调节性T细胞、PCT、NF-κB对老年COPD患者病情变化的诊治提供了重要的实验室依据。Abstract： Objective To observe the changes in proportion of CD4＋ Foxp3 ＋ regulatory T cells （ Treg cell）, procalcitonin （PCT）, nuclear factor kappa-B （nuclear factor-kappa B, NF-KB ） during the elderly patients with acute stage COPD, the elderly patients with stable COPD and in elderly heahhy, to offer clinical information for diagnose and treatment. Methods Forty-two elderly patients with acute stage COPD and thirty-nine elderly patients with stable COPD were investigated as group 1 and group 2, thirty-five elderly healthy were investigated as control group. To detect the proportion of Treg cells, the level of PCT and the percentage of NF-KB 1365. Results （1）the percentage of the Treg cells were significant difference among multiple pairwise comparisons （P 〈 0.05 ） ;（2）the level of PCT was significant difference between the group 1 and group 2, it also was significantly different between the group 1 and the control group（ P 〈 0.05）,but it was no difference between the group 2 and the control group（ P 〉 0.05 ） ;（3）the percentage of the NF-KB were significant difference among multiple pairwise comparisons （ P 〈 O. 05 ）. Conclusion Inflammation mechanism and immunity mechanism both had the important effect in elderly COPD patients. Detecting the change of the Treg cell, NF-KB and PCT in elderly COPD patients provided very important laboratory basis for clinical diagnose and treatment.

关 键 词：CD4+ Foxp3-调节性T细胞 核因子-B 降钙素原 COPD 诱导痰 

分 类 号：R563.8[医药卫生—呼吸系统] R446.62[医药卫生—内科学]