1,25-(OH)_2-D_3对进展性肾炎大鼠肾小管间质胶原蛋白Ⅲ表达的影响  

The effects of 1,25-(OH)_2-D_3 on the expression in tubulointerstitial of ColⅢ in rat with progressive nephritis

作　　者：林建明[1] 周洁[2] 杨霞[1] 达静静[1] 何平红[1] 易韦[3] 查艳[1] 

机构地区：[1]贵州省人民医院肾内科,贵阳550002 [2]贵州省兴义市人民医院肾内科,贵州兴义562400 [3]贵州省人民医院病理科,贵阳550002

出　　处：《中国当代医药》2014年第4期4-6,9,共4页China Modern Medicine

基　　金：国家自然科学基金(81200539);2012年贵州省省长基金(黔省专合字(2012)128号);2010年贵州省优秀科技教育人才省长专项资金项目(黔省专合字(2010)89号);贵州省国际科技合作计划项目(黔科合外G字[2012]7045号)

摘　　要：目的探讨1,25-(OH)2-D3对进展性肾炎大鼠肾小管间质胶原蛋白Ⅲ(ColⅢ)表达的影响。方法将实验动物按随机数字表法分为3组(n=7):假手术组(对照组)、进展性肾炎组(模型组)、6 ng/(100 g·d)1,25-(OH)2-D3干预组(治疗组)。除对照组外,其余组均采用右肾切除并2次注射IgG(OX-7)鼠抗人Thy1.1单克隆抗体,制备进展性肾炎大鼠模型。1,25-(OH)2-D3干预8周后,检测各组大鼠24 h尿蛋白、血肌酐(SCr)、尿素氮(BUN)及电解质的变化,Masson染色观察肾组织病理改变。免疫组织化学检测肾小管间质ColⅢ的表达。结果与模型组比较,1,25-(OH)2-D3治疗不仅使大鼠24 h尿蛋白及BUN均明显降低(P<0.05),还能使ColⅢ表达显著下调(P<0.05)。肾组织病理损害明显减轻。结论 1,25-(OH)2-D3可能通过明显降低进展性肾炎大鼠肾小管间质ColⅢ的表达,减轻肾小管间质纤维化而发挥肾脏保护作用。Objective To investigate the effects of 1,25-（OH）2-D3 on the expression in tubulointerstitial of Col Ill in rat with progressive nephritis. Methods Experimental rats were divided into three groups according to random number table method：sham operation group （control group）,progressive nephritis group （model group）,and progressive nephritis group treated with 6 ng/（100 g-d） 1,25-（OH）2-D3 （treatment group）.In addition to the control group,the other progressive nephritis model was established by right nephrectomy and injection with IgG（OX-7） mouse monoclonal anti-Thyl.1 an- tibody twice.Progressive nephritis rats received 1,25-（OH）2-D3 by intragastric administration for 8 weeks,the urine pro- tein of 24 hours,serum creatinine （SCr）,blood urea nitrogen （BUN）and the levels of calcium and phosphorus in rats of each group were measured.The renal morphologic changes were evaluated on Masson stained sections.The expression in tubulointerstitial of Col 11I was detected by immunohistochemical. Results The 24 hours urine protein and BUN in tubulointerstitial were lower in 1,25-（OH）2-D3 treatment group than those in model group （P〈0.O5）.In addition,the expressions of Collll mRNA in 1,25-（OH）：-D3 treatment group were significantly lower than those in model group （P〈0.05）. The renal pathological damage was significantly reduce. Conclusion 1,25-（OH）2-D3 may protect kidney by de- creasing the expression of tubulointerstitial fibrosis gene Col III in the progressive nephritis model rats,and reduce renal tubular interstitial fibrosis and renal protective effect into full play.

关 键 词：1 25-(OH)2-D ColⅢ 进展性肾炎 肾小管间质纤维化 大鼠 

分 类 号：R692.3[医药卫生—泌尿科学]