桑叶总黄酮不同纯化工艺的比较  

Comparative studies on different methods of purification of total flavones from Mulberry leaf

作　　者：林秀仙[1] 罗爱勤[1] 陈亮[1] 

机构地区：[1]广州白云山汉方现代药业有限公司中药提取分离过程现代化国家工程研究中心,广州510240

出　　处：《中国当代医药》2014年第4期10-11,14,共3页China Modern Medicine

基　　金：广东省科技计划项目(2009A030901008)

摘　　要：目的比较桑叶总黄酮不同的纯化工艺,为找到一种总黄酮含量>15%,简便可行,廉价且适于生产的纯化工艺。方法采用D101型、AB-8型和JD-1型大孔吸附树脂吸附法,乙酸乙酯萃取法,醇提水沉法,醇提-水沉-醇沉法6种方法对桑叶总黄酮进行纯化,以总黄酮的收率和含量为考察指标进行评价。结果 6种方法制备的总黄酮的百分含量依次为:50.3%、48.5%、55.7%、82.1%、10.8%、18.5%,产品收率依次为:2.37%、2.42%、2.28%、0.45%、18.21%、15.93%。结论采用醇提-水沉-醇沉法即可得到符合要求的桑叶总黄酮。Objective To compare the purification effects of different purification techniques of total flavones from Mulberry leaf.In order to find a purification technology which is cheap,simple,feasible and suitable for the production of total flavones whose purity is more than 15%.Methods Six purifying methods,including adsorption with macroreticular adsorption resin D101,AB-8,JD-l,extraction with ethylacetate,alCohol sedimentation method,alcohol sedimentation and alcohol precipitation method,were adopted,and the amound and purity of total flavones collected were compared.Results By using the above-mentioned six methods,the percentages of the contents of total flavones was 50.3%,48.5%,55.7%,82.1%, 10.8% and 18.5% respectively,the recoveries of products was 2.37%,2.42%,2.28%,0.45%,18.21% and 15.93% respective- ly.Conclusion Using the alcohol sedimentation and alcohol precipitation method can get total flavones up to requirement.

关 键 词：桑叶 总黄酮 纯化工艺 

分 类 号：R284[医药卫生—中药学]