新型呼肠病毒M片段对核转录因子κB的活性抑制的研究  

Inhibition of the activation of nuclear factor kappa-light-chain-enhancer of activated B cells by M fragments of new type of reovirus

作　　者：白冰珂[1] 陶玲[2] 沈宏辉[1] 侯俊[1] 胡燕[1] 罗声栋[1] 杨锐创 貌盼勇[1] 

机构地区：[1]解放军第三〇二医院实验技术研究保障中心,北京100039 [2]重庆市中药研究院中药药理毒理研究所,400065

出　　处：《传染病信息》2013年第6期351-354,共4页Infectious Disease Information

基　　金：国家自然科学基金青年基金(81000735)

摘　　要：目的探讨新型呼肠病毒M片段能否抑制核转录因子-κB(nuclear factor kappa-light-chain-enhancer of activated B cells,NF-κB)的活性。方法构建3个不同中基因节段的重组真核表达质粒,并瞬时转染真核细胞,利用荧光素酶双报告系统检测其对NF-κB激活有抑制效应的基因。结果酶切鉴定3个基因重组质粒均构建成功。其中M3基因表达的蛋白对肿瘤坏死因子α介导的NF-κB激活有较明显的抑制,抑制率达55%。而M1和M2基因表达的蛋白较之于空载体均无明显的抑制效果。结论 M3基因能显著抑制肿瘤坏死因子α介导的NF-κB激活,为研究病毒的致病及免疫调控机制提供了重要理论依据。Objective To investigate the effects of M fragments of new type of reovirus on the inhibition of the activation of nuclear factor kappa-light-chain-enhancer of activated B cells （NF-κB）. Methods 293T cells were transfected with different recom-binant plasmids containing M1, M2 and M3 fragments respectively. Then the dual luciferase reporter system was applied to identify the effects of different genes on the inhibition of NF-κB activation. Results Three recombinant plasmids were all constructed suc-cessfully. M3 gene could inhibit tumor necrosis factor （TNF）-α-induced NF-κB activation significantly （55%reduction was detected）. While M1 and M2 genes had no significant inhibitory effect on NF-κB activation. Conclusions M3 gene can inhibit TNF-α-indu-ced NF-κB activation significantly, which provides an evidence for the studies on viral pathogenesis and immunomodulatory properties.

关 键 词：呼肠病毒科 质粒 

分 类 号：R373.1[医药卫生—病原生物学]