急性减压病兔脊髓病变的基因表达谱分析  

Analysis of gene expression profile of pathological changes in spinal cord induced by acute decompression sickness in rabbits

作　　者：刘晓红[1] 高光凯 毛蕊琪[1] 曹永成[1] 秦金燕 毕利泉[1] 时艳辉 耿明[1] 

机构地区：[1]济南军区总医院病理科,济南250031 [2]解放军第四○一医院高压氧科

出　　处：《中华航海医学与高气压医学杂志》2013年第6期384-388,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：全军医学科研“十二五”面上项目资助课题（CWS11J241）

摘　　要：目的 初步探讨急性减压病兔脊髓损伤组织基因表达谱的变化.方法 新西兰大耳白兔10只,5只按文献方法制备急性减压病模型,为急性减压病组;另5只参照空气潜水减压表减至常压,为安全减压组.2组出舱后30 min内各随机选取2只兔,麻醉后解剖,留取胸腰段脊髓,迅速置于液氧中保存.应用基因表达谱芯片对4只兔的脊髓组织进行检测,采用实时定量逆转录聚合酶链反应（reverse transcription polymerase chain reaction,RT-PCR）方法对部分芯片筛选的差异表达基因结果进行验证.结果 芯片杂交结果显示,与安全减压组比较,急性减压病组上调2倍以上的基因9个,下调2倍以上的基因17个,差异表达基因主要涉及炎症、离子通道、细胞周期、物质转运和凋亡等方面.实时定量RT-PCR结果显示,与安全减压组比较,急性减压病组甲状旁腺激素和肿瘤细胞坏死因子分别上调平均6.18倍和6.46倍,酰基辅酶A合成酶和电压门控性离子通道基因分别下调平均2.28倍和3.16倍,与芯片杂交实验结果一致.结论 急性减压病兔脊髓病变差异表达基因主要涉及炎症、细胞周期和凋亡等方面,其意义需要进一步研究.Objective To make a preliminary exploration in the changes of gene expression profile in spinal cord lesion induced by acute decompression sickness in rabbits.Methods Ten New Zealand white rabbits were used for the experiment.Acute decompression sickness model was developed as prescribed in the article in 5 of the animals and were set as the acute decompression sickness group.The other 5 animals were safely decompressed to normal pressure by using the Air Decompression Table,which were set as the safe decompression group.Two rabbits were randomly selected from each group within 30 minutes,when the animals were surfaced out of the chamber.Anatomy was performed after the animals were anesthetized.Sections of thoracic and lumbar spinal cord were taken and preserved in the liquid nitrogen.Gene expression profile chips were used to observe the spinal cord tissues of the 4 rabbits.Selected differential genes contained in some chips were identified with RT-PCR.Results Such were the chip hybridization results：for the acute decompression sickness group,the genes which up-regulated the expressions of genes twice as much were 9,and the genes which down-regulated the expressions of genes twice as much were 17,when comparisons were made with the safe decompression group.Those differential genes were mainly associated with inflammation,ion channels,cell cycle,material transfer and apoptosis,etc.RT-PCR results showed that the expression levels of parathyroid hormone and TNF increased for an average of 6.18 times and 6.46 times respectively for the acute decompression sickness group,when comparisons were made with the safe decompression group.The expression levels of acyl-CoA synthetase and the voltage-gated ion channel gene were down-regulated by an average of 2.28 times and 3.16 times respectively,which was consistent with the experimental Conclusions from chip hybridization,thus confirming the reliability of the research results.Conclusions The differential genes of the involved spinal cord in acute decompression sickness r

关 键 词：高压氧 急性减压病 脊髓损伤 基因表达谱 兔 

分 类 号：R83[医药卫生—航空、航天与航海医学]