波纹唇鱼(Cheilinus undulatus)不同地理种群遗传多样性的微卫星分析  

Genetic Diversity of Different Geographical Populations of Cheilinus undulatus Revealed by Microsatellite Analysis

作　　者：胡静[1,2] 侯新远[1] 尹绍武[1] 祝斐[1] 贾一何[1] 胡亚丽[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210023 [2]中国水产科学研究院南海水产研究所热带水产研究开发中心,广东广州510300

出　　处：《海洋科学进展》2013年第4期538-545,共8页Advances in Marine Science

基　　金：国家自然科学基金项目--波纹唇鱼种质资源的分子遗传多样性研究(40966003);"十一五"国家科技支撑计划重点项目--南海区主要养殖种类种质保存及评价技术(2007BAD29B03)

摘　　要：以海南陵水海域、马来西亚海域、西沙海域、南沙海域四个不同地理群体的波纹唇鱼(Cheilinus undulatus)为研究对象,利用微卫星(SSR)分子标记技术对其群体遗传多样性进行分析。研究结果表明,15对微卫星引物在4个群体中共检测到246个等位基因;期望杂合度、观测杂合度、多态信息含量、Shannon's信息指数结果在4个群体中均以马来西亚群体为最高,分别为0.736、0.531、0.696和1.705;聚类分析结果显示波纹唇鱼的4个群体明显地聚为2支,马来西亚群体、海南陵水群体和西沙群体聚为1支,南沙群体独立1支;AMOVA分子方差分析结果显示,波纹唇鱼60.90%的遗传变异来自于个体间,35.75%的遗传变异来自于群体内个体间,仅有4.15%的遗传变异来自于群体间;群体遗传分化系数Fst为0.373 0,群体间基因流Nm为0.840 5。本研究显示波纹唇鱼种群具有较高遗传多样性水平,群体间存在较大程度的遗传分化。In this study, genetic diversity of the Cheilinus undulatus was analyzed using 15 microsatellite markers among 101 individuals from 4 different geographical populations. The samples were collected from the seawaters of Hainan Lingshui, East Malaysia, Xisha and Nansha, respectively. The results indicated that 246 alleles loci were detected from four populations, and the observed heterozygosity（Ho）, expected heterozygosity（He）, polymorphism information content（PIC） and Shannon（/＋~ ） of Malaysia population were highest which were 0. 736,0. 531,0. 696 and 1. 705, respectively. Phylogenetic tree showed that four populations could be apparently divided into two groups, one group was included three populations of Hainan Lingshui, East Malaysia and Xisha, and the other group was only the Nansha population. The AMOVA demonstrated that 60.90% of genetic variance came from among individuals, 35. 750% of genetic variance came from among individuals within populations and only 4.15% of genetic variance came from a- mong populations. The genetic differentiation coefficient （Fst） and the gene flow （Nm） were 0. 373 0 and 0. 840 5 among four populations. These results indicated that the genetic diversity of C. undulatus was relatively high, and there was genetic differentiation among the four populations of C. undulatus.

关 键 词：波纹唇鱼 遗传多样性 微卫星 

分 类 号：X834[环境科学与工程—环境工程]