杆菌肽脂质体的制备及其药物含量测定  

Preparation and the drug content determination of bacitracin Liposom

作　　者：焦洋[1,2] 尹寿玉[1] 

机构地区：[1]延边大学药学院,吉林延吉133000 [2]北华大学附属医院,吉林吉林132011

出　　处：《中国医药科学》2013年第24期29-30,41,共3页China Medicine And Pharmacy

摘　　要：目的：制备杆菌肽脂质体,并采用HPLC法测定杆菌肽脂质体中杆菌肽含量和包封率。方法用薄膜分散法将杆菌肽制备成杆菌肽脂质体；色谱条件：色谱柱为Diamonsil-C18色谱柱（150mm×4.6mm，5μm）；流动相为pH 6.0的磷酸盐缓冲液-甲醇（60∶40）；柱温为25℃；检测波长为254nm；流速为1mL/min。采用低温离心法分离杆菌肽脂质体中的游离药物。结果所制备的杆菌肽脂质体的平均包封率为87.94%（RSD 1.12%, n=5）；在本色谱条件下，杆菌肽在0.061~1.83μg范围内与峰面积积分值的线性关系良好（R2=0.9997），平均回收率为99.68%~101.38%。结论本HPLC法简单，准确可靠，可用于杆菌肽脂质体中杆菌肽含量及其包封率的测定。Objective To prepare bacitracin liposom,and to determine bacitracin content and entrapment efficiency of the liposome using HPLC method. Methods The bacitracin liposome was prepared by film dispersion method; The chromatographic conditions were Column： Diamonsil-C18 column（150mm×4.6mm,5μm）; mobile phase： pH 6.0 phosphate buffer： methanol （60∶40）; column temperature： 25℃; detection wavelength： 254nm; flow rate： 1mL/min.Refrigerated centrifugation using bacitracin liposomes free drug. Results The mean encapsulation efficiency of the bacitraci liposome was 87.94%（RSD 1.12%,n=5）;In the chromatographic conditions bacitracin and accessories and a good separation of the solvent peak,bacitracin at 0.061-1.83μg range had a good linear relationship （R2=0.9997）,recovery was 99.68%-101.38%. Conclusion The HPLC method is simple, accurate and reliable,can be used to determine bacitracin content and entrapment efficiency of the bacitracin liposom.

关 键 词：杆菌肽脂质体 含量测定 包封率 高效液相色谱法 

分 类 号：R285.1[医药卫生—中药学]