压力下角质形成细胞上清液对成纤维细胞增殖及胶原合成的影响  

Effect of the Supematant of Kerationcytes with Pressure on Fibroblasts Proliferation and Collagen Synthesis

作　　者：邱海霞[1] 刘阳[1] 安美文[1] 许卫玮 冯鹏飞[1] 茹孟洁 

机构地区：[1]太原理工大学应用力学与生物医学工程研究所,山西省材料强度与结构冲击重点实验室,太原030024 [2]太原东方医院,太原030024

出　　处：《生物物理学报》2013年第11期844-852,共9页Acta Biophysica Sinica

基　　金：国家自然科学基金项目(11372208);山西省自然科学基金项目(2013011002-4)~~

摘　　要：探讨人皮肤角质形成细胞(human keratinocytes,HKC)对成纤维细胞(human fibroblasts,HFB)增殖及胶原合成的影响,对于临床治疗增生性瘢痕有重要的理论和实际意义。DMEM与HKC上清液的混合液(1∶1)培养HFB,3.4 kPa气体压力加载5 h或10 h;MTT法测定HFB增殖;羟脯胺酸比色法测定HFB上清液中胶原蛋白合成量。实验结果显示,静态或加压时,HKC组与K-SFM组比较,HFB数量及胶原合成量显著增多(P<0.05);压力组与无压力组比较,HFB数量及胶原合成量显著下降(P<0.05);HKC且加压10 h组与K-SFM且静态10 h组比较,HFB数量显著增多(P<0.05),胶原合成量显著下降(P<0.05)。以上结果说明,HKC上清液可以促进HFB增殖及胶原的合成,3.4 kPa气体压力可以抑制HFB增殖及胶原的合成。Human fibroblasts （HFB） is responsible for treating hypertrophic scars due to their proliferation and the corresponding collagen secretion, which are influenced by human keratinocytes cells （HKC）. This influence was investigated in the present work. HFB were cultured in a mixed solution with 50% HKC supernatant and 50% DMEM. This cultivation process was performed under 3.4 kPa pressure within 5 and 10 h, respectively. In addition, the cultivation without pressure was settled as the control group. MTT method was used to determine the proliferation of HFB, while coloricmetric method was utilized to identify the collagen expression in the supernatant of HFB. The experimental results revealed that HFB proliferation and collagen were increased significantly in the supernatant of HKC groups （P〈0.05） with and without pressure. Compared to the control group, reduction of HFB proliferation and collagen significantly happened in the groups with 3.4 kPa pressure （P〈0.05）. However, in the HKC group under 3.4 kPa pressure for 10 h, HFB proliferation was improved significantly （P〈0.05） but collagen was decreased significantly （P〈0.05）. This investigation suggested that the supernatant of HKC played an important role in improving HFB proliferation and collagen, which was inhibited by 3.4 kPa pressure.

关 键 词：压力 角质形成细胞 成纤维细胞 胶原蛋白 

分 类 号：Q274[生物学—细胞生物学]