轻龄和老龄红细胞表面Zeta电位测量条件的研究  

Measurement Conditions of Surface Membrane Zeta Potential for Young and Old RBCs

作　　者：吴正洁[1] 王成[2] 康立丽[3] 

机构地区：[1]北京理工大学珠海学院化工与材料学院,广东珠海519088 [2]仲恺农业工程学院信息科学与技术学院,广州510225 [3]南方医科大学生物医学工程学院,广州510515

出　　处：《生物物理学报》2013年第11期853-862,共10页Acta Biophysica Sinica

基　　金：国家质检总局科技计划项目(2010QK094)~~

摘　　要：红细胞表面的电学特性与其结构功能密切相关,而表面Zeta电位是衡量细胞表面电荷的重要参数。针对以往研究对轻、老龄红细胞表面电荷差异性的争议,利用Percoll密度梯度离心法分离出轻、老龄红细胞,从细胞表面形态和测量溶液的离子强度两个方面,认为测量溶液的选择不当,会使红细胞变成棘形,导致所测表面Zeta电位降低。其次,溶液的离子强度与红细胞表面Zeta电位成反比关系,在满足细胞"扩散双电子层"形成的条件下,应选择离子强度低的溶液,如0.0145 mol/L NaCl+0.026 mol/L萄萄糖溶液,作为红细胞悬浮液用于细胞表面Zeta电位的测量,测出轻龄红细胞的Zeta电位值为(-32.46±1.24)mV,老龄红细胞的Zeta电位为(-25.88±1.39)mV,后者的负电位比前者少了近20%,其测量结果能准确地反映出轻、老龄红细胞间表面电荷的差异。The surface electrical characteristics of RBCs are closely related to its structural functions, and the Zeta potential is the crucial parameter when measuring the RBC＇s membrane surface charge. Owing to the uncertainty of the difference between young and old RBC＇s surface membrane Zeta potential, in this study, Percoll density gradient separation method was used to separate the young and old RBCs. By comparing the cell morphology and the ionic strength of suspension solution, it was pointed out that the RBC＇s surface Zeta potential was found to be influenced by the shape of the cells. RBCs would become echinocytes if the cell suspension solution is not suitable, the surface membrane Zeta potential would be also decreased at the same time. Secondly, the RBC＇s surface Zeta potential was inversely proportional to the ionic strength of suspension solution. Using 0.0145 mol/L NaCl ＋ 0.026 mol/L glucose as the suspension solution, it was found that the Zeta potential of the young RBC was （-32.46 ± 1.24） mV, and the old one was （-25.88 ± 1.39） mV. The negative potential of the old one was about 20% less than the young one, indicating that it enables accurate detection of the surface membrane Zeta potential of living RBCs.

关 键 词：红细胞 细胞年龄 表面电荷 ZETA电位 

分 类 号：Q952.7[生物学—动物学]