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作 者:杜文婷[1,2] 韩宝龙[1] 刘亚妮[1] 李建文[1] 李军[1] 杨官娥[1]
机构地区:[1]山西医科大学药学院,山西太原030001 [2]寿阳县第二人民医院,山西寿阳035400
出 处:《现代药物与临床》2014年第1期36-39,共4页Drugs & Clinic
基 金:国家自然科学基金资助项目(81173473);山西省自然科学基金资助项目(2010011048-2);太原市科技项目人才专项明星专题项目(120247-08);山西医科大学青年基金(02201128;02201124)
摘 要:目的考察枸杞提取液对球形红细菌生长的影响。方法在不同质量浓度的常规培养基中添加不同质量浓度的枸杞提取液,培养球形红细菌,比较球形红细菌的活菌数、生长曲线和脱氢酶活性,考察枸杞提取液对球形红细菌生长的影响。结果在培养基中添加枸杞提取液可缩短球形红细菌生长延迟期,使其提前进入指数期和稳定期;当质量浓度为12.5 g/L时,球形红细菌活菌数增加到之前的1.5倍,使球形红细菌脱氢酶活性提高到之前的3.3倍。结论培养基中添加一定量的枸杞提取液可以促进球形红细菌的生长,使球形红细菌生长周期缩短、活力提高。Objective To study the effects ofLycium barbarum extract on the growth ofRhodobacter sphaeroides. Methods Adding L. barbarum extract into general culture medium at different concentration, the effects ofL. barbarum extract on the growth of R. sphaeroides were investigated by culturing R. sphaeroide and comparing living R. sphaeroides bacteria number, growth curve, and dehydrogenase activity.. Results After adding L. barbarum extract into the culture medium, the growth delay period of R. sphaeroides was shortened and the expotential and stationary periods were advanced. When the concentration ofL. barbarum extract was 12.5 g/L, the number of living R. sphaeroides was increased in 1.5 times, and the dehydrogenase activity ofR. sphaeroides was improved in 3.3 times as much as before. Conclusion When L. barbarum extract is added into the culture medium, the growth and the activity ofR. sphaeroides could be improved, and the ~owth cycle could be shortened.
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