雌激素补充时机对绝经模型大鼠空间学习能力和记忆力的影响  

Effects of estrogen replacement therapy on cognitive function of ovariectomized rats

作　　者：刘晓燕[1] 王丽平[1] 彭丹红[1] 王艳[1] 任慕兰[1] 

机构地区：[1]南京东南大学附属中大医院妇产科,210009

出　　处：《中华妇产科杂志》2014年第1期42-47,共6页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的观察雌激素补充时机对绝经模型大鼠空间学习和记忆力的影响，为绝经后雌激素补充时机的选择提供动物实验依据。方法将40只SD大鼠行双侧卵巢切除术，建立绝经模型。术后将模型大鼠计算机随机分为早期激素补充治疗（HRT）组（n=10，术后3d予戊酸雌二醇补充治疗30d）、早期对照组（n=10，术后3d予生理盐水30d）、晚期HRT组（n=10，术后90d予戊酸雌二醇补充治疗30d）、晚期对照组（n：10，术后90d予生理盐水30d）。对40只大鼠行Morris水迷宫实验测定行为学指标；并行MRI质子波谱成像技术扫描检测海马神经元代谢产物相关数据，包括N-乙酰基天冬氨酸（NAA）、胆碱化合物（Cho）、肌酸复合物（Cr）、肌醇（mI），并以cr值为标准，计算NAA／Cr、Cho／Cr、mI／Cr、NAA／mI比值。结果（1）Morris水迷宫定位航行实验中，逃避潜伏期：早期对照组为（43±13）s，早期HRT组为（28±9）S，晚期对照组为（82±26）S，晚期HRT组为（48±18）s；游泳行程：早期对照组（1404±238）em，早期HRT组（878±354）em，晚期对照组（2411±818）em，晚期HRT组（1310±434）em；绝经模型大鼠早、晚期HRT组的逃避潜伏期及游泳行程明显较早、晚期对照组短（P〈0．叭）。（2）Morris水迷宫空间探索实验：早期HRT组在目标象限游泳停留时间为（34．0±3．0）S，较其他各组时间长（P〈0．05）。（3）MRI质子波谱成像技术检测结果：NAA／Cr：早期对照组为1．12±0．17，早期HRT组1．26±0．12，晚期对照组1．57±0．21，晚期HRT组1．38±0．28，晚期HRT组和晚期对照组的NAAZCr高于早期HRT组和早期对照组（F=6．05，P=0．040）；早期HIlT组与早期对照组、晚期HIlT组与晚期对照组比较，差异无统计学意义（F=0．04，P=0．860）。mI／Cr：早期对照组0．69±0．04，早期ttRT组0．46±0．12，晚期对照组0．70±0．03，晚期ttRT组Objective To investigate the effect of estrogen replacement therapy applied in different periods to ovariectomized rats and to evaluate the cognitive function of the rats. Methods Totally 40 rats were ovariectomized to be postmenopausal models. They were divided into early hormone replacement therapy group （n = 10, managed by estradiol valerate at day 3 after surgery） , early control group （n = 10, managed by saline at day 3 after surgery）, late hormone replacement therapy （ n = 10, managed by estradiol valerateat day 90 after surgery for 30 days） and late control group （n = 10, managed by saline at day 90 after surgery for 30 days ）. The behavior indicators of the rats were evaluated by Morris watermaze and hippocampal metabolite was detected by proton magnetic resonance spectroscopy, including N-acetylaspartate （NAA）, choline containing compounds （Cho）, creatine（Cr）, myoinositol （mI）, NAA/Cr, Cho/Cr and mI/Cr. Results （ 1 ）Navigation test： escape latency were （43 ± 13） s at the early control group, （28 ± 9） s at the early HRT group, （82±26） s at the late control group and （48 ± 18） s at late HRT group. Swimming distance were （ 1 404 ± 238） cm at the early control group, （878 ± 354） cm at the early HRT group, （2 411±818） cm at the late control group and （1 310 ±434） cm at the late HRT group. The escape latency and swimming distance of the early and late HRT groups were significantly shorter than those at the control groups （ P 〈 0. 05 ）. （2） Spatial probe test ： the swimming time in the target quadrant of rats in the early HRT group （ 34. 0 ± 3.0） s were longer than those in other groups （ P 〈 0. 05 ）. （ 3 ） Proton magnetic resonance spectroscopy： NAA/Cr were 1.12 ±0. 17 at the early control group, 1.26 ±0. 12 at the early HRT group, 1.57 ±0. 21 at the late control group and 1.38 ±0. 28 at the late HRT group. The late HRT group and the late control group were higher than their early groups（ F = 6. 05,

关 键 词：绝经期 雌激素替代疗法 学习 记忆 模型 动物 

分 类 号：R711.75[医药卫生—妇产科学] R-332[医药卫生—临床医学]