VEGF165b在结直肠癌组织中的差异表达及其意义  

Clinical significance of differential expression of VEGF 165b between colorectal cancer and normal intestinal mucosa tissues

作　　者：赵一军[1] 梁勇[2] 石忱长[1] 韩华中[1] 朱庆超[1] 杨俊[1] 

机构地区：[1]上海交通大学附属上海市第六人民医院普外科,上海市200233 [2]徐州医学院附属医院普外科,江苏省徐州市221002

出　　处：《世界华人消化杂志》2014年第2期273-278,共6页World Chinese Journal of Digestology

摘　　要：目的:研究血管内皮生长因子165b(vascular endothelial growth factor 165b,VEGF 165b)在结直肠癌(colorectal cancer,CRC)组织及其配对的正常黏膜组织中的表达情况,探讨其与结直肠癌发生与发展的关系.方法:用实时荧光定量逆转录聚合酶链反应(real-time quantitative reverse transcriptase polymerase chain reaction,real-time qRT-PCR)分别检测20对结直肠癌组织及其配对的正常肠黏膜组织中VEGF 165b mRNA的表达情况;运用统计学方法对结直肠癌组织与正常黏膜组织、浆膜内侧与浆膜外侧组织、无淋巴结转移与有淋巴结转移组织以及TNM分期Ⅱ期与Ⅲ期组织中的VEGF 165b mRNA的表达情况统计与分析.结果:VEGF l65b在结直肠癌组织中的表达明显低于正常黏膜组织的表达,统计具有显著性差异(P=0.006);在浆膜内侧与浆膜外侧组织、无淋巴结转移与有淋巴结转移组织以及TNM分期Ⅱ期与Ⅲ期组织中的VEGF 165b mRNA的表达无明显差别,统计无显著性差异(P>0.05).结论:VEGF 165b在结直肠癌组织中的表达明显低于正常黏膜组织的表达,提示VEGF165b可能具有抗血管生成、抑制肿瘤生长的作用,为结直肠癌的诊断、治疗和预后提供新的靶点.AIM： To investigate the differential expression of vascular endothelial growth factor （VEGF） 165b between colorectal cancer （CRC） and normal intestinal mucosa tissues and its role in the development of CRC. METHODS： Real-time qRT-PCR was performed to detect the expression of VEGF 165b mRNA in 20 fresh CRC tissues and 20 matched normal intestinal mucosa tissues. Statistical methods were used to compare the expression of VEGF 165b in CRC tissues and normal intestinal mucosa tissues; tissues inside and outside the serosal layer; tissues with and without lymph node metastasis; tissues of TNM stages Ⅱ and Ⅲ tumors. RESULTS： The positive expression rate of VEGF l65b mRNA in normal intestinal mucosa tissues was significantly higher than that in CRC tissues （P = 0.006）. The expression of VEGF 165b was not statistically significant between CRC tissues and normal intestinal mucosa tissues, between tissues inside and outside the serosal layer, between tissues with and without lymph node metastasis, or tissues between TNM stages Ⅱ and Ⅲ tumors. CONCLUSION： VEGF 165b might play a role in inhibiting the development of CRC and provide a new target for the diagnosis, prognosis and treatment of this malignancy.

关 键 词：结直肠癌 选择性剪接 VEGF 165b 实时 荧光定量逆转录聚合酶链反应 

分 类 号：R735.3[医药卫生—肿瘤]