糖尿病大鼠主动脉平滑肌细胞原代培养方法的改良  

Improvement of Method for Primary Culture of Vascular Smooth Muscle Cells of Diabetic Rats

作　　者：杨婷[1] 金松[1] 刘彦东[1] 

机构地区：[1]佳木斯大学附属第一医院血管外科,黑龙江佳木斯154000

出　　处：《中国普外基础与临床杂志》2014年第2期173-176,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：2013年度黑龙江省自然科学基金项目(项目编号:H201358)~~

摘　　要：目的探寻一种简便、高效的糖尿病大鼠主动脉平滑肌细胞体外原代培养的方法,建立长久稳定的体外糖尿病血管平滑肌细胞模型,为研究糖尿病血管慢性病变奠定基础。方法采用链脲佐菌素腹腔注射加高脂高糖饲养自制糖尿病Wister大鼠模型20只,采用改良酶消化法体外培养主动脉平滑肌细胞。结果成功建立糖尿病大鼠模型,用改良酶消化法体外培养的血管平滑肌细胞传代生长快,细胞存活率达96%,能满足进行体外细胞实验要求。结论与传统方法相比,改良酶消化法简单、经济、成活率高。Objective To explore a simple, efficient method for primary culture in vascular smooth muscle cells of diabetic rat, to establish a long-term stable diabetic vascular smooth muscle cell model in vitro, and lay the foundation for the study of diabetes chronic vascular lesions. Methods Twenty diabetic Wister rats models were self-made by using streptozotocin intraperitoneal injection plus high fat and sugar feeding, the vascular smooth muscle cells in vitro was cultured by a modified enzymatic digestion. Results The diabetic rat models were successfully established, the vascular smooth muscle cells cultured in vitro by modified enzyme digestion grew fast, the cell survival was 96%, it could meet the requirements for cell experiment in vitro. Conclusion Comparing with the traditional method, the modified enzymatic di^zestion method is simlole, economic, hi^zh survival rate.

关 键 词：糖尿病大鼠 主动脉平滑肌细胞 体外原代培养 

分 类 号：R587.1[医药卫生—内分泌]