武汉地区痤疮患者皮损中分离的丙酸杆菌耐药性分析  

Antimicrobial Susceptibility of Propionibacteria Isolated from Acne Patients in Wuhan Area

作　　者：曾宪玉[1,2] 姜敏[1] 付辰[1] 吴卓璇[1] 刘雯[1] 董碧麟[1] 曾志良[1] 王玮蓁[1] 

机构地区：[1]武汉市第一医院皮肤科,湖北武汉430022 [2]湖南中医药大学,湖南长沙410208

出　　处：《中国皮肤性病学杂志》2014年第2期131-134,137,共5页The Chinese Journal of Dermatovenereology

基　　金：湖北省感染性皮肤病临床医学研究中心专项基金(2009BCC11)

摘　　要：目的观察从痤疮患者皮损中分离出的丙酸杆菌对红霉素和四环素的药敏试验结果。方法从痤疮患者皮损中分离丙酸杆菌，厌氧培养获得分离株，经革兰染色、16SrRNA和23SrRNA测序等方法鉴定，采用E—test方法检测分离株对红霉素和四环素的敏感性。结果共分离84株丙酸杆菌，经革兰染色，镜下可见革兰染色阳性的棒状杆菌。所有分离株16SrRNA和23SrRNA扩增序列Blast结果显示：72株与Genbank中痤疮丙酸杆菌（Propionibacterium0c，Pacbe16SrRNA和23SrRNA的序列有98％-100％的同源性，12株与Genbank中贪婪丙酸杆菌（Propionibacteriumavidum，Pavidum）16SrRNA和23SrRNA的序列有99％～100％的同源性。84株分离株中，所有菌株对四环素敏感，MIC值在0．016—0．50μg/mL间。29株（34．52％）对红霉素耐药，均为高度耐药（MIC〉256μg/mL）；其余55株对红霉素敏感（MIC值0．016—0．38μg/mL）。P0cnes和P.avidum中红霉素耐药株分别为19株（26．39％）、10株（83．33％）。结论武汉地区痤疮患者携带的丙酸杆菌对红霉素耐药率高，对四环素均敏感。该药敏试验结果可指导临床合理应用抗生素。Objective To detect the susceptibility of propionibacteria isolated from ache patients to erythromycin and tetracycline. Methods Samples were collected from acne lesions of 84 acne patients. All samples were cul- tured in anaerobic conditions. The identification of isolates was performed by gram stain and sequencing of 16s rRNA and 23s rRNA. Isolates of propionibacteria were subjected to antimicrobial susceptibility tests using erythromycin and tetracycline with E-test. Results Seventy-two propionibacteria acnes and 12 propionibacte- ria avidum were isolated respectively. Isolates resistant to erythromycin（29/84,34.52% ） were observed and all isolates were susceptible to tetracycline. Isolates resistant to erythromycin were observed in P. aches （26. 39%, 19/72） and P. avidttm （83.33%, 10/12） respectively. 37.70% of isolates taken from patients with a history of antibiotic use demonstrated resistance, whereas 26.09% of isolates taken from patients who had never used antibiotics demonstrated resistance. Conclusion Antimicrobial resistance in propionibacteria in this Wuhan population has a higher prevalence than those reported in Japan and Korea and follows a simi- lar pattern to findings in Europe. No isolates were resistant to tetracycline.

关 键 词：丙酸杆菌 痤疮 贪婪丙酸杆菌 耐药 红霉素 四环素 

分 类 号：R758.73[医药卫生—皮肤病学与性病学]