山西地区淋球菌质粒谱型分析  

Analysis on Plasmid Profiles of Neisseria Gonorrhoeae in Shanxi Province

作　　者：叶峰山[1] 李桢[2] 李玉串[3] 崔雪萍[2] 李连青[2] 

机构地区：[1]厦门大学附属第一医院,福建厦门361022 [2]山西省临床检验中心,山西太原030012 [3]太原市传染病医院,山西太原030012

出　　处：《中国皮肤性病学杂志》2014年第2期154-155,共2页The Chinese Journal of Dermatovenereology

基　　金：山西省科技攻关项目(20100311103)

摘　　要：目的了解山西地区淋球菌流行株的质粒谱,探讨淋球菌质粒谱与耐药谱间的关系。方法采用碱裂解法提取山西地区54株淋球菌的质粒,分析其质粒谱型。结果 54株淋球菌中,51株有可靠的分析结果,其中49株检测出携带有质粒,检出率96.08%。以7.4kb+4.2kb,39.5kb+7.4kb+4.2kb及42.5kb+39.5kb+7.4kb+4.2kb三种类型居多,占82.35%。结论质粒图谱显示山西地区的质粒谱型有别于国内其他地区,具有地区特殊性,产青霉素酶淋球菌(PPNG)与7.4kb质粒密切相关。Objective To survey plasmid profiles of Neisseria gonorrhoeaes in Shanxi, probe the relationship between the plasmid profiles and resistance of neisseria gonorrhoeaes. Methods The plasmids were extracted by al kaline lysis technique. Analysis the plasmid profiles of neisseria gonorrhoeaes in Shanxi. Resuts Fifty-one strains of fifty-four strains has the reliable resuhs. The detection rate of plasmid is 96.08%. Three main types were 7.4kb ＋ 4.2kb, 39.5kb ＋ 7.4kb ＋ 4.2kb and 42.5kb ＋ 39.5kb ＋ 7.4kb ＋ 4.2kb accounting for 82.35% of total isolates. Conclusion The plasmid profiles of neisseria gonorrhoeaes in Shanxi are different of the other areas. It has its specificity. PPNG was correlated to the 7.4 kb plasmid.

关 键 词：淋球菌 质粒 山西 

分 类 号：R759.2[医药卫生—皮肤病学与性病学]