河南省番茄黄化曲叶病病原分子鉴定及全基因组序列分析  

Molecular Identification and Complete Sequence Analysis of the Pathogen Causing Tomato Yellow Leaf Curl Disease in Henan Province

作　　者：于云奇[1] 阮涛[1] 杨水英[1] 周常勇[1,2] 青玲[1,2] 

机构地区：[1]西南大学植物保护学院植物病害生物学重庆市高校级重点实验室,重庆400716 [2]中国农业科学院柑橘研究所,重庆400712

出　　处：《西南大学学报（自然科学版）》2014年第1期13-17,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30971897)

摘　　要：利用双生病毒简并引物PA/PB对采自河南中牟县13份表现为黄化、曲叶症状的番茄样品进行PCR检测,结果发现11份样品检测呈阳性,检出率为84.6%.选取样品HN-158,利用滚环扩增PCR(RCA-PCR)、克隆、测序等技术获得病毒基因组DNA-A全序列,该DNA分子全长为2 781nts(登录号JQ004028.1),与已报道的番茄黄花曲叶病毒Tomato yellow leaf curl virus,TYLCV山东泰安分离物SDTA(NCBI登录号:JF414236.1)核酸序列相似度最高,为99.8%.进化分析表明,该病毒分离物与来自菏泽、泰安、济南、石家庄及天津等地的TYLCV分离物亲缘关系较近.以上结果表明中牟县田间番茄黄化曲叶病样品均受到TYLCV侵染,该病毒分离物可能来源于我国山东番茄上的TYLCV.Thirteen tomato samples showing stunting, yellowing and leaLcurling symptoms were collected from Zhongmu of Zhengzhou, Henan province in 2011. The degenerated primer pair PA/PB was used to detect the geminiviruses by PCR. Of the 13 samples examined, 11 were shown to be positive, the detection rate being 84.6%. With the techniques of rolling circle amplification and PCR （RCA-PCR）, cloning and sequencing, a complete nucleotide sequence of HN-158 DNA-A was successfully obtained, and identified to be 2781 nts in size （Genbank accession number JQ004028.1）. Sequence analysis revealed that HN- 158 DNA-A shared the highest nucleotides identity （99.8%） with that of the isolate tomato yellow leaf curl virus [TYLCV-SDTA] （Genbank accession number JF414236.1）, which is from Taian of Shandong province. Phylogenetic tree showed that HN-158 DNA A was closely clustered together with TYLCV iso- lates from Heze, Taian, Jinan, Shijiazhuang and Tianjin. These results further suggested that these toma- to samples with typical symptoms in Zhongmu county were infected with TYLCV which was presumably transmitted from Shandong Province through insect vectors.

关 键 词：河南省 番茄 番茄黄化曲叶病 番茄黄化曲叶病毒 

分 类 号：S436.412.1’1[农业科学—农业昆虫与害虫防治]