上调血红素氧合酶1对高血压合并心肌梗死后心力衰竭的作用  

Effect of upregulated heme oxygenase-1level on heart failure in hypertensive rats with myocardial infarction

作　　者：陈天萌[1,2] 曹剑[1] 陈薇 李德斌 石海燕[1] 李健[1] 李小鹰[1] 范利[1] 

机构地区：[1]解放军总医院南楼心血管一科,北京100853 [2]南开大学医学院 [3]总后北京老干部服务局门诊部 [4]总后北京老干部服务局总参保健处

出　　处：《中华老年心脑血管病杂志》2014年第1期15-20,共6页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81270154);北京市自然科学基金(7122175)

摘　　要：目的研究上调血红素氧合酶1(HO-1)对高血压合并心肌梗死大鼠模型血压及心功能的影响。方法选择同为13周龄的雄性自发性高血压大鼠40只及雄性Wistar大鼠20只,随机分为6组,假手术组、对照组、高血压组、模型组、诱导剂组(钴原卟啉4.5mg/kg)、抑制剂组(锡中卟啉15mg/kg),每组10只。术后第1天开始给药,腹腔注射,1次/周,持续6周后,进行相关指标的检测。结果与模型组比较,诱导剂组的血压显著降低,心肌梗死面积缩小,LVEF、左心室短轴缩短率、左心室压力最大上升和下降速率/左心室收缩压峰值升高;左心室舒张末内径、左心室收缩末内径和全心重/体质量下降;血清C反应蛋白、白细胞介素6水平降低,血清总胆红素、一氧化氮、前列环素水平升高(P<0.05,P<0.01)。与诱导剂组比较,抑制剂组血压与心功能的改善情况被阻断。结论上调HO-1能通过抑制炎性反应、抑制左心室重构、改善内皮功能紊乱等机制,降低高血压合并心肌梗死大鼠模型的血压,提高梗死后心功能。Objective To study the effect of upregulated heme oxygenase 1 （HO-I） level on blood pressure and cardiac function in hypertensive rats with myocardial infarction. Methods Sixty male hypertensive Wistar rats aged 13 weeks were divided into sham operation group, control group,hypertension group,model group,inducer group and inhibitor group （10 in each group）. CoPP （4.5 mg/kg） and SnMP （15 mg/kg）were injected into their abdomen,once a week for 6 weeks,and their different parameters were then detected. Results The blood pressure,LVEF,left ventricular pressure,falling rate/peak left ventricular systolic pressure value ratio, the serum lev- els of TB,NO and PGI2 were significantly higher, the myocardial infaction area was significantly smaller,the left ventricular mior axis was significantly shorter, the left ventricular diastolic di- mension and systolic end dimension and whole heart weight/body weight ratio were significantly lower,and the serum levels of C reactive protein and IL-6 were significantly lower in inducer group than in model group. The blood pressure and cardiac function were poorer in inhibitor group than in inducer group. Conclusion Upregulated HO-1 level can decrease the blood pressure and improve the cardiac function in hypertensive rats with myocardial infarction by inhibiting in- flammatory reaction and left ventricular remolding,and improving endothelial functiobn.

关 键 词：血红素加氧酶-1 心力衰竭 C反应蛋白质 高血压 

分 类 号：R542.22[医药卫生—心血管疾病] R544.1[医药卫生—内科学]