A novel fluorescent detection for PDGF-BB based on dsDNA-templated copper nanoparticles  

作　　者：Xiao-Hai Yang Shan Sun Pei liu Ke-Min Wang Qing Wang Jian-Bo Liu Jin Huang Lei-Liang He 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Key Laboratory for Bio-nanotechnology and Molecular Engineering of Hunan Province

出　　处：《Chinese Chemical Letters》2014年第1期9-14,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.21190041,21190044,21175035);National Basic Research Program(No.2011CB911002);Interna-tional Science&Technology operation Program of China(No.2010DFB30300)

摘　　要：A novel method for the detection of PDGF-BB has been developed using double-strand DNA-copper nanoparticles （dsDNA-CuNPs） as fluorescent markers. This assay relies on the premise that the aptamer- based probe undergoes a conformational change upon binding with target protein, and subsequently triggers polymerization reaction to generate dsDNA. Then, the resultant dsDNA can be used as a template for the formation of CuNPs with high fluorescence. Under the optimized conditions, the proposed assay allowed sensitive and selective detection of PDGF-BB with a detection limit of 4 nmol/L. This possibly makes it an attractive platform for the detection of a variety of biomolecules whose aptamers undergo similar conformational change.A novel method for the detection of PDGF-BB has been developed using double-strand DNA-copper nanoparticles （dsDNA-CuNPs） as fluorescent markers. This assay relies on the premise that the aptamer- based probe undergoes a conformational change upon binding with target protein, and subsequently triggers polymerization reaction to generate dsDNA. Then, the resultant dsDNA can be used as a template for the formation of CuNPs with high fluorescence. Under the optimized conditions, the proposed assay allowed sensitive and selective detection of PDGF-BB with a detection limit of 4 nmol/L. This possibly makes it an attractive platform for the detection of a variety of biomolecules whose aptamers undergo similar conformational change.

关 键 词：Copper nanoparticles PDGF-BB Polymerization 

分 类 号：O657.3[理学—分析化学]