Electrospray ionization tandem mass spectrometric studies to probe the interaction of Cu(II) with amoxicillin  

作　　者：Ramaiyan Sekar Suresh Kumar Kailasa Yuan-Chin Chen Hui-Fen Wu 

机构地区：[1]Department of Chemistry,National Sun Yat Sen University,Kaohsiung,804 [2]Depatment of Chemistry,S. V. National Institute of Technology,Surat,395007,India [3]Department of Chemistry,Tamkang University,New Taipei [4]School of Pharmacy,College of Pharmacy,Kaohsiung Medical University,Kaohsiung,806 [5]Doctoral Degree Program in Marine Biotechnology,National Sun Yat-Sen University,Kaohsiung,804 [6]Center for Nanoscience and Nonotechnoloty,National Sun Yat-Sen University

出　　处：《Chinese Chemical Letters》2014年第1期39-45,共7页中国化学快报（英文版）

基　　金：National Science Council(NSC)forfinancial support

摘　　要：This study describes the application of electrospray ionization mass spectrometry （ESI-MS） to investigate copper ion interaction with amoxicillin. ESI mass spectra of Cu-amoxicillin complexes show complex ions at m/z 828, 792, 753, 731,428, 388 and 366 corresponding to [63Cu＋（2A-H）＋2H2O]＋, [63Cu＋（2A-H）]＋, [2A＋Na]＋, [2A＋H]＊, [63Cu＋（A-H）]~, [A＋Na]~ and [A＋H]＋ （where A = amoxicillin）. Based on the observed m/z values of Cu-amoxicillin complex ions, it is found that the Cu-amoxicillin ratios are 1：1 and 1：2, and the copper ions exhibited three feasible coordination numbers （2, 4 and 6） with arnoxicillin complexes. The structures and coordination numbers of copper-arnoxicillin complex ions were probed from their collisionally activated dissociation （CAD） spectra. Based on these results, it is confirmed that the copper ions could form stable tetrahedral and octahedral complexes with amoxicillin. This study validates the annlicabilitv of ESI-MS for nrobin; CODDer-amoxicillin comDlex ions.This study describes the application of electrospray ionization mass spectrometry （ESI-MS） to investigate copper ion interaction with amoxicillin. ESI mass spectra of Cu-amoxicillin complexes show complex ions at m/z 828, 792, 753, 731,428, 388 and 366 corresponding to [63Cu＋（2A-H）＋2H2O]＋, [63Cu＋（2A-H）]＋, [2A＋Na]＋, [2A＋H]＊, [63Cu＋（A-H）]~, [A＋Na]~ and [A＋H]＋ （where A = amoxicillin）. Based on the observed m/z values of Cu-amoxicillin complex ions, it is found that the Cu-amoxicillin ratios are 1：1 and 1：2, and the copper ions exhibited three feasible coordination numbers （2, 4 and 6） with arnoxicillin complexes. The structures and coordination numbers of copper-arnoxicillin complex ions were probed from their collisionally activated dissociation （CAD） spectra. Based on these results, it is confirmed that the copper ions could form stable tetrahedral and octahedral complexes with amoxicillin. This study validates the annlicabilitv of ESI-MS for nrobin; CODDer-amoxicillin comDlex ions.

关 键 词：Amoxicillin Copper ion ESI-MS 

分 类 号：O657.63[理学—分析化学] R914[理学—化学]