多途径介导的小胶质细胞间钙波传递  

Multi-Pathways-Mediated Propagations of Intercellular Calcium Waves in Microglial Cells

作　　者：吴限[1] 潘雷霆[1] 刘颖[1] 邢梦溪 张心正[1] 许京军[1] 

机构地区：[1]南开大学物理科学学院,泰达应用物理研究院,弱光非线性光子学教育部重点实验室,天津300457

出　　处：《生物化学与生物物理进展》2014年第2期179-184,共6页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(11204142);国家重点基础研究发展计划(2010CB934101);高等学校博士学科点专项科研基金(20110031120004);国家基础科学人才培养基金(J1103208);高等学校学科创新引智计划(B07013)资助项目~~

摘　　要：利用微局域机械力刺激,快速实时观察机械力引起的细胞间钙波传递,系统地研究了BV-2小胶质细胞间钙通讯机制.结果表明,在细胞种植密度较小且彼此未接触的情况下,旁分泌途径可介导BV-2小胶质细胞间钙波传递.在细胞密度较大且相互接触的情况下,旁分泌和间隙连接两种途径可共同介导胞间钙波传递.更为有趣的是,在体外发现BV-2小胶质细胞间存在通道纳米管类似物连接,也可介导小胶质细胞间钙波传递.综上所述,小胶质细胞间钙波传递可通过旁分泌、间隙连接和通道纳米管类似物连接三种途径介导.In the present work, we applied mechanical stimulus to study the propagations of intercellular calcium waves among BV-2 microglial cells by the fast fluorescence microscopy imaging system in vitro. The results showed that calcium signaling responded to mechanical stimulus and propagated to neighboring cells in isolated BV-2 microglial cells, suggesting that the paracrine pathway mediated intercellular calcium waves. Further data indicated that gap junction was involved in mechanically-induced intercellular calcium waves when the cells had physical contact with each other. More importantly, tunneling nanotubes （TNTs）-like structure between BV-2 microglial cells was found in vitro and mediated the propagation of intercellular calcium wave. Taken together, our studies demonstrated that three distinct pathways, including paracrine, gap junction and TNTs-like structures, could mediate the intercellular calcium waves in BV-2 microglial cells.

关 键 词：BV-2小胶质细胞 细胞间钙波 间隙连接 旁分泌 通道纳米管类似物 

分 类 号：Q632[生物学—生物物理学] Q274