温和灸对动脉粥样硬化兔Th1/Th2比例失衡的影响及其调节规律  

Effects of mild moxibustion on imbalance of Th1/Th2 in rabbits with atherosclerosis and its regulating rules

作　　者：吴姗[1] 王玲玲[1] 蔡海红[1] 蒋文[1] 

机构地区：[1]南京中医药大学针药结合实验室,江苏南京210029

出　　处：《中国针灸》2014年第2期163-167,共5页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究发展计划(973)项目:2009 CB 522905

摘　　要：目的:观察温和灸法及药物洛伐他汀对慢性高脂血症并动脉粥样硬化家兔模型免疫功能的影响,初步阐明温和灸对免疫功能的调节规律。方法:将32只日本雄性大耳白兔随机分出8只作为空白组,其余24只均采用内皮损伤并高脂饲料饲养家兔方法制备动脉粥样硬化模型。空白组普通饲料喂养,自由饮水;另24只造模10周后再随机分为模型组、艾灸组、药物组,每组8只。艾灸组于"神阙""后三里"穴给予每天每穴10min艾灸治疗;药物组采用洛伐他汀胶囊以每天3.6mg/kg灌胃给药。治疗结束取血清,用生化比色法测定血清总胆固醇(TC)、高密度脂蛋白(HDL-C),评价动脉粥样硬化指数(AI);用酶联免疫吸附法测定γ干扰素(IFN-γ)、白细胞介素4(IL-4)。结果:①模型组血清胆固醇(TC)、高密度脂蛋白(HDL-C)均高于空白组、艾灸组与药物组,且差异均有统计学意义(均P<0.01),艾灸组AI(1.683±0.486)远低于模型组(20.301±4.022,P<0.01);②艾灸组、药物组Th1/Th2比值均显著低于模型组(0.569±0.143vs 0.917±0.255,0.445±0.079vs 0.917±0.255,均P<0.01),而艾灸组与药物组相比,差异无统计学意义(P>0.05)。结论:艾灸干预动脉粥样硬化能够通过降低动脉粥样硬化指数,影响辅助性T细胞的漂移偏向,调整机体体液免疫与细胞免疫的平衡,使人体恢复到相对免疫平衡的状态,从而减缓了动脉粥样硬化发生发展以及血栓破裂进程。Objective To observe effects of mild moxibustion and lovastatin on immunologic function in rabbits with chronic hyperlipidaemia and atherosclerosis （AS） to initially explain regulating rules of mild moxibustion on immunologic function. Methods Among thirty-two Japanese male big-ear rabbits, 8 rabbits were randomly selected as a blank group, the rest 24 rabbits were fed with method of endothelial injury and high-fat diet to establish AS model. The blank group was raised with normal diet and free water. After ten weeks of model establishment, the rest 24 rabbits were randomly divided into a model group, a moxibustion group and a medicine group, eight rabbits in each one. Moxibustion was applied at ＂Shenque＂ （CV 8） and ＂Zusanli＂ （ST 36） for 10 min per acupoint per day in the moxibustion group, while intragastric administration of 3.6 mg/kg lovastatin capsule was applied in the medicine group. After treatment, serum was acquired. Spectrophotometry method was adapted to measure cholesterol （TC） and high-density lipoprotein （HDL-C） and evaluated atherosclerosis index （AI）, while enzymelinked immunosorbent assay method was used to measure interferon-γ（IFN-γ） and interleukin-4 （IL-4）. Results （1）The serum TC and HDL-C in the model group were significantly higher than those in the blank group, moxibustion group and medicine group （all P〈0.01）, The mean value of AI was 1. 683±0. 486 in the moxibustion group, which was obviously lower than 20. 301±4. 022 in the model group （P〈0.01）. （2）The ratio of Th1/Th2 was 0. 569±0. 143 in the moxibustion group and 0. 445 ±0. 079 in the medicine group, which were significantly lower than 0. 917±0. 255 in the model group （both P〈0. 01）, but there was no statistically significant difference between the moxibustion group and the medicine group （P〈0. 05）. Conclusion The moxibustion for AS could reduce atherosclerosis index, influence drift and bias of helper T cell and regulate balance between humoral immunity an

关 键 词：动脉粥样硬化 温和灸 免疫平衡 

分 类 号：R245.8[医药卫生—针灸推拿学]