乌司他丁对甘油诱导横纹肌溶解大鼠IL-6、TNF-α释放的影响及其对横纹肌的保护作用  

Effect of ulinastatin on the release of IL-6 and TNF-α in rats with rhabdomyolysis induced by glycerol and its protective effect on striated muscle

作　　者：潘志国[1] 邵玉[2] 陈怿[1] 陈镜合[3] 苏磊[1] 

机构地区：[1]广州军区广州总医院重症医学科,全军热区创伤救治与组织修复重点实验室,广州510010 [2]广州军区广州总医院华侨科,广州510010 [3]广州中医药大学第一附属医院内科,广州510405

出　　处：《解放军医学杂志》2014年第2期105-108,共4页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科学技术研究"十二五"发展计划重点项目(BWS12J018);国家自然科学基金(81071529;81101467;81101406)~~

摘　　要：目的研究乌司他丁对甘油诱导横纹肌溶解大鼠血浆白细胞介素6(IL-6)和肿瘤坏死因子(TNF-α)的影响及其对横纹肌的保护作用。方法 24只Wistar大鼠随机分为4组,每组6只。正常对照组;模型组:肌注50%甘油8ml/kg;低剂量乌司他丁组和高剂量乌司他丁组:造模后分别静脉给予1000U和5000U乌司他丁,1次/12h。72h后立即行下腔静脉采血检测大鼠血浆IL-6、TNF-α水平,并分离大鼠右后肢横纹肌行病理及电镜切片检查。结果造模后大鼠血浆IL-6、TNF-α水平均较正常对照组明显升高(P<0.05)。低剂量乌司他丁组血IL-6、TNF-α水平分别为528.97±77.34、584.65±53.22pg/ml,高剂量乌司他丁组分别为860.68±45.32、480.45±49.78pg/ml,与正常对照组(分别为1546.14±106.33、414.52±55.19pg/ml)及模型组(分别为1994.09±99.45、822.93±78.23pg/ml)比较均明显下降(P<0.05)。病理切片及电镜观察结果显示,乌司他丁组横纹肌组织损伤较模型组明显减轻,其中高剂量乌司他丁组横纹肌肌纹基本恢复正常。结论乌司他丁可抑制甘油诱导横纹肌溶解大鼠血浆IL-6和TNF-α的释放,并对机体横纹肌具有保护作用。Objective To investigate the effect of ulinastatin on the levels of serum IL-6 and TNF-α in rats with rhabdomyolysis induced by glycerol, and its protective effect on striated muscle. Methods Twenty-four rats were randomly divided into 4 groups （6 each）： normal control group, model group with intramuscular injection of 8ml/kg glycerol, low-dose and high-dose treatment group with intravenous injection of 1000 and 5000U ulinastatin respectively, once per 12h. The rats were sacrificed 72h after treatment. The levels of serum IL-6 and TNF-α were determined, and pathological changes in striated muscle in the affected limb were examined under electron microscope. Results The serum IL-6 and TNF-α levels were 528.97 ± 77.34, 584.65 ±53.22 in low-dose treatment group, and 860.68± 45.32, 480.45 ±49.78 in high-dose treatment group, which were significantly lower than those in normal control group （1546.14 ± 106.33, 414.52±55.19 respectively） and in model group （1994.09 ± 99.45, 822.93± 78.23 respectively, P〈0.05）. The pathological examination showed that the changes in striated muscle was milder in both treatment groups than in model group. Conclusion Ulinastatin can inhibit the release of serum IL-6 and TNF-α and protect the striated muscle from impairment in rats with rhabdomyolysis induced by glycerol.

关 键 词：乌司他丁 横纹肌溶解 炎症 

分 类 号：R685.5[医药卫生—骨科学]