桃红四物汤调节血管内皮细胞功能及治疗动脉硬化闭塞症的实验研究  

Taohong Siwu Decoction Regulated Functions of Endothelial Cells and Treated Arteriosclerosis Obliterans:an Experimental Study

作　　者：李润生[1,2] 李大勇[1] 陈文娜[3] 马贤德[3] 张扬[1] 李雪晶[1] 

机构地区：[1]辽宁中医药大学附属医院血管外科,沈阳110032 [2]大连大学中山医院血管外科,辽宁大连116001 [3]辽宁中医药大学教学实验中心,沈阳110847

出　　处：《中国中西医结合杂志》2014年第2期191-196,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81173273);辽宁省自然科学基金资助项目(No.201202154);辽宁省高等学校优秀人才支持计划项目(No.LJQ2012083);沈阳市科学技术项目(No.F12-277-1-73)

摘　　要：目的探讨桃红四物汤调节血管内皮细胞功能、治疗动脉硬化闭塞症(arteriosclerosis obliterans,ASO)的作用。方法采用高脂饮食饲喂及动脉内膜损伤的方法制作大鼠ASO模型,随机分为正常组(10只)、模型组(9只)、桃红四物汤低浓度组(中药A组,12只)、桃红四物汤中浓度组(中药B组,10只)、桃红四物汤高浓度组(中药C组,9只),造模后8周用激光多普勒血流仪观测肢体血流灌注,光镜、透射电镜观察动脉大体形态,Percoll密度梯度离心法测定循环内皮细胞(circulating endothelial cells,CECs)数量,酶联免疫吸附(ELISA)双抗体夹心法检测血清中肿瘤坏死因子-α(TNF-α)、IL-1、内皮素-1(ET-1)和NO水平。结果成功建立了大鼠ASO模型,模型组大鼠血脂水平明显升高,左后肢血流灌注量明显下降,外周血中CECs数量明显增多,动脉管腔不规则狭窄,血管壁超微结构被破坏,大鼠血清TNF-α、IL-1、ET-1水平均明显升高,NO水平明显降低,与正常组比较,差异有统计学意义(P<0.01)。与模型组比较,中药B组和中药C组的上述各项指标均有明显改善,差异有统计学意义(P<0.05,P<0.01)。结论内皮细胞损伤及分泌功能的异常是ASO发生的重要病理过程,桃红四物汤能够保护血管内皮细胞,改善血管内皮细胞的分泌功能,是ASO的有效治疗方药。ABSTRACT Objective To discuss the effect of Taohong Siwu Decoction （TSD） in regulating func- tions of endothelial cells and treating arteriosclerosis obliterans （ASO）. Methods The ASO model was prepared by using high-fat diet plus intimal injury. They were randomly divided into the model group （n = 10）, the normal control group （n =9）, the low dose TSD group （group A, n =12）, the middle dose TSD group （group B, n =10）, and the high dose TSD group （group C, n =9）. Eight weeks after modeling, the limb blood perfusion was observed using laser Doppler flowmetry. The arterial morphology was observed u- sing light microscope and transmission electron microscope. The number of circulating endothelial cells （CECs） was determined using Percoll density gradient centrifugation method. Serum levels of TNF-α, IL-1, ET-1, and NO were detected using double antibody sandwich assay of enzyme linked immunosorbent assay （ELISA）. Results The ASO rat model was successfully established. Blood lipids levels significantly in- creased, the blood perfusion of left hind limbs significantly decreased, the number of CECs in the peripher- al blood significantly increased, the arterial lumen was irregularly narrowed, the ultra-structure of vessel walls was damaged, serum levels of TNF-α, IL-1, and ET-1 significantly increased, and the serum level ofNO significantly decreased in the model group, showing statistical difference when compared with the nor- mal control group （P 〈0.01 ）. Compared with the model group, significant improvement in the aforesaid in- dices was shown in group B and C （P 〈0.05,P 〈0.01 ）. Conclusions The injury and abnormal functions of endothelial cells is an important pathological process of ASO. As an effective recipe for treating ASO, TSD could protect vascular endothelial cells and improve the secretion function of vascular endothelial cells.

关 键 词：桃红四物汤 动脉硬化闭塞症 内皮细胞 循环内皮细胞 

分 类 号：R259[医药卫生—中西医结合]